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Literature summary extracted from

  • Fuhshuku, K.; Asano, Y.
    Synthesis of (R)-beta-nitro alcohols catalyzed by R-selective hydroxynitrile lyase from Arabidopsis thaliana in the aqueous-organic biphasic system (2011), J. Biotechnol., 153, 153-159.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.10 synthesis synthesis of (R)-beta-nitro alcohols using nitromethane as donor. Highest enantioselectivity is obtained with n-butyl acetate as solvent with an optimum aqueous phase content of 50% (v/v) Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.2.10 butyl acetate synthesis of (R)-beta-nitro alcohols, highest enantioselectivity is obtained with n-butyl acetate as solvent with an optimum aqueous phase content of 50% (v/v) Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Arabidopsis thaliana
-
-
-
4.1.2.10 Arabidopsis thaliana Q9LFT6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10 ammonium sulfate precipitation and Ni Sepharose column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Arabidopsis thaliana cyanide + benzaldehyde
-
 ?
4.1.2.10 2-chlorobenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(2-chlorophenyl)-2-nitroethanol 34% yield after 2 h ?
4.1.2.10 2-methoxybenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(2-methoxyphenyl)-2-nitroethanol 13% yield after 2 h ?
4.1.2.10 2-methylbenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(2-methylphenyl)-2-nitroethanol 12% yield after 2 h ?
4.1.2.10 3-(2-naphthyl)benzaldehyde + nitromethane
-
 Arabidopsis thaliana (1R)-1-[3-(naphthalen-2-yl)phenyl]-2-nitroethanol 7% yield after 2 h ?
4.1.2.10 3-chlorobenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(3-chlorophenyl)-2-nitroethanol 17% yield after 2 h ?
4.1.2.10 3-methoxybenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(3-methoxyphenyl)-2-nitroethanol 17% yield after 2 h ?
4.1.2.10 3-methylbenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(3-methylphenyl)-2-nitroethanol 12% yield after 2 h ?
4.1.2.10 4-bromobenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(4-bromophenyl)-2-nitroethanol 20% yield after 2 h ?
4.1.2.10 4-chlorobenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(4-chlorophenyl)-2-nitroethanol 9% yield after 2 h ?
4.1.2.10 4-fluorobenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(4-fluorophenyl)-2-nitroethanol 9% yield after 2 h ?
4.1.2.10 4-methoxybenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(4-methoxyphenyl)-2-nitroethanol 2% yield after 2 h ?
4.1.2.10 4-methylbenzaldehyde + nitromethane
-
 Arabidopsis thaliana 1-(4-methylphenyl)-2-nitroethanol 11% yield after 2 h ?
4.1.2.10 benzaldehyde + nitromethane
-
 Arabidopsis thaliana (R)-2-nitro-1-phenylethanol 30% yield after 2 h ?
4.1.2.10 nitromethane + 2-chlorobenzaldehyde
-
 Arabidopsis thaliana (1R)-1-(2-chlorophenyl)-2-nitroethanol 34% yield, 68% enantiomeric excess ?
4.1.2.10 nitromethane + 3-methoxybenzaldehyde
-
 Arabidopsis thaliana (1R)-1-(3-methoxyphenyl)-2-nitroethanol 17% yield, 91% enantiomeric excess ?
4.1.2.10 nitromethane + 4-fluorobenzaldehyde
-
 Arabidopsis thaliana (1R)-1-(4-fluorophenyl)-2-nitroethanol 20% yield, 81% enantiomeric excess ?
4.1.2.10 nitromethane + benzaldehyde 30% yield, 91% enantiomeric excess Arabidopsis thaliana (1R)-2-nitro-1-phenylethanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 R-HNL
-
 Arabidopsis thaliana
4.1.2.10 R-selective hydroxynitrile lyase
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 7
-
-
 Arabidopsis thaliana