EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.99.14 | gene CYP509C12, cloning from a cDNA library, DNA and amino acid sequence determination and analysis, phylogenetic analysis, and recombinant expression in Pichia pastoris, coexpression with NAD(P)H-dependent reductase also from Rhizopus oryzae, which improves electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone | Rhizopus arrhizus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.99.14 | additional information | gene CYP509C12, production of a recombinant Pichia pastoris engieered to coexpress gene CYP509C12, ecoding the enzyme with the NAD(P)H-dependent reductase also from Rhizopus oryzae, to improve electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone and is useful for biotechnologic applications | Rhizopus arrhizus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.14 | 11-deoxycorticosterone + AH2 + O2 | Rhizopus arrhizus | - |
? + A + H2O | - |
? | |
1.14.99.14 | 11-deoxycorticosterone + AH2 + O2 | Rhizopus arrhizus RA 99-880 | - |
? + A + H2O | - |
? | |
1.14.99.14 | 11-deoxycortisol + AH2 + O2 | Rhizopus arrhizus | - |
? + A + H2O | - |
? | |
1.14.99.14 | 11-deoxycortisol + AH2 + O2 | Rhizopus arrhizus RA 99-880 | - |
? + A + H2O | - |
? | |
1.14.99.14 | progesterone + AH2 + O2 | Rhizopus arrhizus | - |
11alpha-hydroxyprogesterone + A + H2O | - |
? | |
1.14.99.14 | progesterone + AH2 + O2 | Rhizopus arrhizus RA 99-880 | - |
11alpha-hydroxyprogesterone + A + H2O | - |
? | |
1.14.99.14 | testosterone + AH2 + O2 | Rhizopus arrhizus | - |
11alpha-hydroxytestosterone + A + H2O | - |
? | |
1.14.99.14 | testosterone + AH2 + O2 | Rhizopus arrhizus RA 99-880 | - |
11alpha-hydroxytestosterone + A + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.14 | Rhizopus arrhizus | - |
gene CYP509C12 | - |
1.14.99.14 | Rhizopus arrhizus RA 99-880 | - |
gene CYP509C12 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.14 | 11-deoxycorticosterone + AH2 + O2 | - |
Rhizopus arrhizus | ? + A + H2O | - |
? | |
1.14.99.14 | 11-deoxycorticosterone + AH2 + O2 | - |
Rhizopus arrhizus RA 99-880 | ? + A + H2O | - |
? | |
1.14.99.14 | 11-deoxycortisol + AH2 + O2 | - |
Rhizopus arrhizus | ? + A + H2O | - |
? | |
1.14.99.14 | 11-deoxycortisol + AH2 + O2 | - |
Rhizopus arrhizus RA 99-880 | ? + A + H2O | - |
? | |
1.14.99.14 | additional information | hydroxylations take place predominantly at 11alpha and 6beta positions of the steroid ring system | Rhizopus arrhizus | ? | - |
? | |
1.14.99.14 | additional information | hydroxylations take place predominantly at 11alpha and 6beta positions of the steroid ring system | Rhizopus arrhizus RA 99-880 | ? | - |
? | |
1.14.99.14 | progesterone + AH2 + O2 | - |
Rhizopus arrhizus | 11alpha-hydroxyprogesterone + A + H2O | - |
? | |
1.14.99.14 | progesterone + AH2 + O2 | - |
Rhizopus arrhizus RA 99-880 | 11alpha-hydroxyprogesterone + A + H2O | - |
? | |
1.14.99.14 | testosterone + AH2 + O2 | - |
Rhizopus arrhizus | 11alpha-hydroxytestosterone + A + H2O | - |
? | |
1.14.99.14 | testosterone + AH2 + O2 | - |
Rhizopus arrhizus RA 99-880 | 11alpha-hydroxytestosterone + A + H2O | - |
? |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.99.14 | Rhizopus arrhizus | CYP509C12 expression is induced by progesterone | up |