Literature summary extracted from
Ujita, M.; Yamanaka, M.; Maeno, Y.; Yoshida, K.; Ohshio, W.; Ueno, Y.; Banno, Y.; Fujii, H.; Okumura, H.
Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures (2011), J. Biosci. Bioeng., 111, 22-25.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.2.1.28 |
synthesis |
expression as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system. Only N-terminally tagged trehalase shows a high activity |
Bombyx mori |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.28 |
- |
Bombyx mori |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.28 |
validoxylamine A |
complete inhibition |
Bombyx mori |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.28 |
0.35 |
- |
alpha,alpha-trehalose |
pH 5.4, 37°C, recombinant protein with N-terminal His-tag |
Bombyx mori |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.28 |
68000 |
- |
x * 68000, recombinant enzyme with His-tag |
Bombyx mori |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.28 |
Bombyx mori |
- |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.2.1.28 |
glycoprotein |
recombinant protein with N-terminal His-tag contains O-linked oligosaccharide chains having Galbeta1-3GalNAc-structure without terminal sialic acids |
Bombyx mori |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.28 |
recombinant protein |
Bombyx mori |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.2.1.28 |
144.7 |
- |
pH 5.4, 37°C, recombinant protein with N-terminal His-tag |
Bombyx mori |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.28 |
alpha,alpha-trehalose + H2O |
- |
Bombyx mori |
2 D-glucose |
- |
? |
|
3.2.1.28 |
additional information |
no substrate: maltose, isomaltose, laminaribiose, cellobiose, sucrose, lactose |
Bombyx mori |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.28 |
? |
x * 68000, recombinant enzyme with His-tag |
Bombyx mori |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.28 |
5.5 |
- |
recombinant protein with N-terminal His-tag |
Bombyx mori |