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Literature summary extracted from

  • Kwon, H.J.; Yeom, S.J.; Park, C.S.; Oh, D.K.
    Substrate specificity of a recombinant D-lyxose isomerase from Providencia stuartii for monosaccharides (2010), J. Biosci. Bioeng., 110, 26-31.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.15 food industry the enzyme is an industrial producer of D-lyxose Providencia stuartii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.15 expressed in Escherichia coli ER2566 cells Providencia stuartii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.15 Ba2+ about 80% residual activity at 1 mM Providencia stuartii
5.3.1.15 Co2+ about 15% residual activity at 1 mM Providencia stuartii
5.3.1.15 Cu2+ about 55% residual activity at 1 mM Providencia stuartii
5.3.1.15 EDTA 12% residual activity at 1 mM Providencia stuartii
5.3.1.15 Fe2+ about 60% residual activity at 1 mM Providencia stuartii
5.3.1.15 additional information not affected by Ca2+ Providencia stuartii
5.3.1.15 Zn2+ about 60% residual activity at 1 mM Providencia stuartii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.15 22
-
 D-mannose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 38
-
 D-xylulose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 47
-
 D-Lyxose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 54
-
 D-fructose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.15 Mg2+ about 150% activity at 1 mM Mg2+ Providencia stuartii
5.3.1.15 Mn2+ maximal activity (about 250%) in the presence of 1 mM Mn2+ Providencia stuartii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.15 22000
-
 2 * 22000, SDS-PAGE Providencia stuartii
5.3.1.15 22160
-
 2 * 22160, calculated from amino acid sequence Providencia stuartii
5.3.1.15 44000
-
 gel filtration Providencia stuartii

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.15 Providencia stuartii
-
-
-
5.3.1.15 Providencia stuartii KCTC 2568
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.15 His-Trap column chromatography, gel filtration Providencia stuartii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.15 0.002
-
 using L-tagatose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.003
-
 using L-mannose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.005
-
 using D-psicose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.007
-
 using L-lyxose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.01
-
 using L-psicose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.011
-
 using L-talose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.013
-
 using D-allose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.014
-
 using D-ribose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.015
-
 using D-tagatose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.017
-
 using L-fructose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.022
-
 using L-allose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.045
-
 using D-tagatose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.096
-
 using D-ribulose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.18
-
 using L-xylulose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.219
-
 using L-ribose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.403
-
 using D-fructose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.523
-
 using D-mannose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 0.872
-
 using L-ribulose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 22.33
-
 using D-lyxose as substrate, at pH 7.5 and 45°C Providencia stuartii
5.3.1.15 24.04
-
 using D-xylulose as substrate, at pH 7.5 and 45°C Providencia stuartii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.15 D-allose
-
 Providencia stuartii D-psicose
-
 r
5.3.1.15 D-allose
-
 Providencia stuartii KCTC 2568 D-psicose
-
 r
5.3.1.15 D-fructose
-
 Providencia stuartii D-mannose
-
 r
5.3.1.15 D-Lyxose highest specific activity Providencia stuartii D-Xylulose
-
 r
5.3.1.15 D-Mannose
-
 Providencia stuartii D-Fructose
-
 r
5.3.1.15 D-ribose
-
 Providencia stuartii D-ribulose
-
 r
5.3.1.15 D-ribose
-
 Providencia stuartii KCTC 2568 D-ribulose
-
 r
5.3.1.15 D-talose
-
 Providencia stuartii D-tagatose
-
 r
5.3.1.15 D-xylulose
-
 Providencia stuartii D-lyxose
-
 r
5.3.1.15 L-allose
-
 Providencia stuartii L-psicose
-
 r
5.3.1.15 L-lyxose
-
 Providencia stuartii L-xylulose
-
 r
5.3.1.15 L-lyxose
-
 Providencia stuartii KCTC 2568 L-xylulose
-
 r
5.3.1.15 L-Mannose
-
 Providencia stuartii L-Fructose
-
 r
5.3.1.15 L-Mannose
-
 Providencia stuartii KCTC 2568 L-Fructose
-
 r
5.3.1.15 L-ribose
-
 Providencia stuartii L-ribulose
-
 r
5.3.1.15 L-talose
-
 Providencia stuartii L-tagatose
-
 r
5.3.1.15 additional information the enzyme does not isomerizes D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate Providencia stuartii ?
-
 ?
5.3.1.15 additional information the enzyme does not isomerizes D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate Providencia stuartii KCTC 2568 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.15 homodimer 2 * 22000, SDS-PAGE Providencia stuartii
5.3.1.15 homodimer 2 * 22160, calculated from amino acid sequence Providencia stuartii

Synonyms

EC Number Synonyms Comment Organism
5.3.1.15 D-Lyxose isomerase
-
 Providencia stuartii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.15 45
-
-
 Providencia stuartii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.15 40 50 at 40°C and 50°C, the activities are approximately 60% of the maximum Providencia stuartii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.15 30 50 the enzyme shows half-lives of 36 h, 14 h, 8.9 h, 3.4 h, and 1.4 h at 30°C, 35°C, 40°C, 45°C, and 50 °C, respectively Providencia stuartii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.15 2010
-
 D-fructose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 2640
-
 D-mannose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 31340
-
 D-Lyxose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 34960
-
 D-xylulose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.15 7.5
-
-
 Providencia stuartii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.15 7 8.5 at pH 7.0 and 8.5, the activities are approximately 60% of the maximum Providencia stuartii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.15 37
-
 D-fructose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 116
-
 D-mannose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 669
-
 D-Lyxose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii
5.3.1.15 920
-
 D-xylulose in 50 mM HEPES buffer, at pH 7.5, 45°C Providencia stuartii