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Literature summary extracted from

  • Tongsook, C.; Sucharitakul, J.; Thotsaporn, K.; Chaiyen, P.
    Interactions with the substrate phenolic group are essential for hydroxylation by the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase (2011), J. Biol. Chem., 286, 44491-44502.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.9
-
 Acinetobacter baumannii

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.9 H120D mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate Acinetobacter baumannii
1.14.14.9 H120E mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate Acinetobacter baumannii
1.14.14.9 H120K catalyzes hydroxylation with efficiency comparable to that of the wild-type enzyme, the hydroxylation rate constant for H120K is 5.7 per s and the product conversion ratio is 75%, compared to values of 16 s-1 and 90% for the wild-type enzyme Acinetobacter baumannii
1.14.14.9 H120N mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate Acinetobacter baumannii
1.14.14.9 H120Q mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate Acinetobacter baumannii
1.14.14.9 H120R mutant is able to catalyze hydroxylation Acinetobacter baumannii
1.14.14.9 H120Y mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate Acinetobacter baumannii
1.14.14.9 S146A product formation is pH-independent and constant at about 70% over a pH range of 6-10 Acinetobacter baumannii
1.14.14.9 S146C product formation decreases from about 65% at pH 6.0 to 27% at pH 10.0 Acinetobacter baumannii

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.9 Acinetobacter baumannii Q6Q272
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.9 3-hydroxyphenylacetate + FMNH2 + O2
-
 Acinetobacter baumannii 3,4-hydroxyphenylacetate + FMN + H2O hydroxylation rate constant is 16 per s and the product conversion ratio is 90% ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.9 HPA 3-hydroxylase
-
 Acinetobacter baumannii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.9 additional information
-
 hydroxylation reaction of wildtype oxygenase component is pH-independent between pH 6 and 10 Acinetobacter baumannii