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Literature summary extracted from
- Intracellular erythrocyte PAF acetylhydrolase 1 inactivates aspirin in blood (2011), J. Biol. Chem., 286, 34820-34829.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.47 | expression of PAFAH1B2 or PAFAH1B3 subunits in HEK-293T cells | Homo sapiens |
| 3.1.1.55 | expression of PAFAH1B2 or PAFAH1B3 subunits in HEK-293T cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.47 | methoxy arachidonyl fluorophosphonate | type 1 PAF acetylhydrolase is irreversibly inhibited | Homo sapiens |  |
| 3.1.1.47 | NaF | - |
Homo sapiens | |
| 3.1.1.47 | platelet activating factor | competitive | Homo sapiens | |
| 3.1.1.55 | methoxy arachidonyl fluorophosphonate | type 1 PAF acetylhydrolase is irreversibly inhibited | Homo sapiens | |
| 3.1.1.55 | NaF | - |
Homo sapiens | |
| 3.1.1.55 | platelet activating factor | competitive | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.47 | additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
| 3.1.1.47 | 2.1 | - |
platelet activating factor | native enzyme, pH 7.2, 37°C | Homo sapiens | |
| 3.1.1.55 | additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
| 3.1.1.55 | 1.13 | - |
Acetylsalicylic acid | recombinant catalytic subuit PAFAH1B2, pH 7.2, 37°C | Homo sapiens | |
| 3.1.1.55 | 2.17 | - |
Acetylsalicylic acid | native enzyme, pH 7.2, 37°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.47 | intracellular | - |
Homo sapiens | 5622 | - |
| 3.1.1.55 | intracellular | - |
Homo sapiens | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 29000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 3.1.1.47 | 30000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 3.1.1.47 | 45000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 3.1.1.47 | 70000 | - |
native enzyme, gel filtration | Homo sapiens |
| 3.1.1.55 | 29000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 3.1.1.55 | 30000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 3.1.1.55 | 45000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 3.1.1.55 | 70000 | - |
native enzyme, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.47 | additional information | Homo sapiens | the type 1 PAF acetylhydrolase is one of three mammalian family members of the group 7 phospholipase A2 family, a family that is unique in that all members require a short sn-2 ester such as the acetyl ester of PAF | ? | - |
? | |
| 3.1.1.47 | platelet activating factor + H2O | Homo sapiens | erythrocyte and plasma PAF acetylhydrolases | acetate + deacetylated platelet activating factor | - |
? | |
| 3.1.1.55 | acetylsalicylic acid + H2O | Homo sapiens | aspirin is hydrolyzed inside the erythrocytes, not in plasma | acetate + salicylate | - |
? | |
| 3.1.1.55 | additional information | Homo sapiens | the type 1 PAF acetylhydrolase is one of three mammalian family members of the group 7 phospholipase A2 family, a family that is unique in that all members require a short sn-2 ester such as the acetyl ester of PAF | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.47 | Homo sapiens | - |
- |
- |
| 3.1.1.55 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.47 | native enzyme 1400fold from erythrocytes by two different steps of anion exchange chromatography, followed by gel filtration, recombinant PAFAH1B2 or PAFAH1B3 subunits from HEK-293T cells | Homo sapiens |
| 3.1.1.55 | native enzyme 1400fold from erythrocytes by two different steps of anion exchange chromatography, followed by gel filtration, recombinant PAFAH1B2 or PAFAH1B3 subunits from HEK-293T cells | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.47 | erythrocyte | erythrocyte aspirin hydrolysis varies among individuals | Homo sapiens | - |
| 3.1.1.55 | erythrocyte | erythrocyte aspirin hydrolysis varies among individuals | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.47 | additional information | the type 1 PAF acetylhydrolase is one of three mammalian family members of the group 7 phospholipase A2 family, a family that is unique in that all members require a short sn-2 ester such as the acetyl ester of PAF | Homo sapiens | ? | - |
? | |
| 3.1.1.47 | additional information | recombinant PAFAH1B2, but not its family member plasma PAF acetylhydrolase, hydrolyzes acetylsalicylic acid to acetate and salicylate, activity of EC 3.1.1.55 | Homo sapiens | ? | - |
? | |
| 3.1.1.47 | platelet activating factor + H2O | erythrocyte and plasma PAF acetylhydrolases | Homo sapiens | acetate + deacetylated platelet activating factor | - |
? | |
| 3.1.1.55 | acetylsalicylic acid + H2O | aspirin is hydrolyzed inside the erythrocytes, not in plasma | Homo sapiens | acetate + salicylate | - |
? | |
| 3.1.1.55 | acetylsalicylic acid + H2O | activity of recombinant PAFAH1B2, but not its family member plasma PAF acetylhydrolase, hydrolyzes acetylsalicylic acid | Homo sapiens | acetate + salicylate | - |
? | |
| 3.1.1.55 | additional information | the type 1 PAF acetylhydrolase is one of three mammalian family members of the group 7 phospholipase A2 family, a family that is unique in that all members require a short sn-2 ester such as the acetyl ester of PAF | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.47 | More | type I PAF acetylhydrolase is a trimer of a noncatalytic 45 kDa protein subunit associated with two catalytic subunits, PAFAH1B3 and PAFAH1B2 as homo- or heterodimers | Homo sapiens |
| 3.1.1.47 | trimer | 1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 3.1.1.55 | More | type I PAF acetylhydrolase is a trimer of a noncatalytic 45 kDa protein subunit associated with two catalytic subunits, PAFAH1B3 and PAFAH1B2 as homo- or heterodimers | Homo sapiens |
| 3.1.1.55 | trimer | 1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.47 | aspirin hydrolase | - |
Homo sapiens |
| 3.1.1.47 | More | type I PAF acetylhydrolase is a structurally unrelated member of this small family of group VII phospholipases A2 | Homo sapiens |
| 3.1.1.47 | PAF acetylhydrolase | - |
Homo sapiens |
| 3.1.1.47 | PAF acetylhydrolase I | - |
Homo sapiens |
| 3.1.1.47 | PAFAH | - |
Homo sapiens |
| 3.1.1.47 | plasma PAF acetylhydrolase | - |
Homo sapiens |
| 3.1.1.47 | type I PAF acetylhydrolase | - |
Homo sapiens |
| 3.1.1.47 | type I platelet-activating factor acetylhydrolase | - |
Homo sapiens |
| 3.1.1.55 | aspirin hydrolase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 37 | - |
assay at | Homo sapiens |
| 3.1.1.55 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 7.2 | - |
assay at | Homo sapiens |
| 3.1.1.55 | 7.2 | - |
assay at | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.47 | 0.29 | - |
platelet activating factor | recombinant catalytic subunit PAFAH1B2, pH 7.2, 37°C | Homo sapiens | |
| 3.1.1.47 | 0.47 | - |
platelet activating factor | native enzyme, pH 7.2, 37°C | Homo sapiens | |
| 3.1.1.55 | 0.29 | - |
platelet activating factor | recombinant catalytic subuit PAFAH1B2, pH 7.2, 37°C | Homo sapiens | |
| 3.1.1.55 | 0.47 | - |
platelet activating factor | native enzyme, pH 7.2, 37°C | Homo sapiens | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.47 | physiological function | type I platelet-activating factor acetylhydrolase is a phospholipase A2 with selectivity for acetyl residues of PAF, and acts as aspirin acetylhydrolase. The intracellular erythrocyte PAF acetylhydrolase 1 inactivates aspirin in blood, and is the major aspirin hydrolase of human blood | Homo sapiens |
| 3.1.1.55 | physiological function | type I platelet-activating factor acetylhydrolase is a phospholipase A2 with selectivity for acetyl residues of PAF, and acts as aspirin acetylhydrolase. The intracellular erythrocyte PAF acetylhydrolase 1 inactivates aspirin in blood, and is the major aspirin hydrolase of human blood | Homo sapiens |
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