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Literature summary extracted from

  • Godinic-Mikulcic, V.; Jaric, J.; Hausmann, C.D.; Ibba, M.; Weygand-Durasevic, I.
    An archaeal tRNA-synthetase complex that enhances aminoacylation under extreme conditions (2011), J. Biol. Chem., 286, 3396-3404.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11 co-expression of MtSerRS with arginyl-tRNA synthetase, MtArgRS, EC 6.1.1.19, from in the Saccharomyces cerevisiae two-hybrid system using yeast strain MaV203 Methanothermobacter thermautotrophicus
6.1.1.19 expression of GST-tagged MtArgRS in Escherichia coli strain BL21(DE3), co-expression of MtArgRS with seryl-tRNA synthetase, MtSerRS, EC 6.1.1.11, from in the Saccharomyces cerevisiae two-hybrid system using yeast strain MaV203 Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 additional information
-
 additional information addition of MtArgRS to MtSerRS leads to an almost 4fold increase in the catalytic efficiency of serine attachment to tRNA, also under conditions of elevated temperature and osmolarity, but has no effect on the activity of MtArgRS, steady-state kinetic analyses, overview Methanothermobacter thermautotrophicus
6.1.1.11 0.002
-
 tRNASer pH 7.5, 50°C, recombinant MtSerRS in presence of recombinant MtArgRS Methanothermobacter thermautotrophicus
6.1.1.11 0.00561
-
 tRNASer pH 7.5, 50°C, recombinant MtSerRS Methanothermobacter thermautotrophicus
6.1.1.19 additional information
-
 additional information addition of MtArgRS to MtSerRS leads to an almost 4fold increase in the catalytic efficiency of serine attachment to tRNA, also under conditions of elevated temperature and osmolarity, but has no effect on the activity of MtArgRS, steady-state kinetic analyses, overview Methanothermobacter thermautotrophicus
6.1.1.19 0.00466
-
 tRNAArg pH 7.5, 50°C, recombinant MtArgRS Methanothermobacter thermautotrophicus
6.1.1.19 0.00477
-
 tRNAArg pH 7.5, 50°C, recombinant MtArgRS in presence of recombinant MtSerRS Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.11 Mg2+ required Methanothermobacter thermautotrophicus
6.1.1.19 Mg2+ required Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer Methanothermobacter thermautotrophicus
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 additional information Methanothermobacter thermautotrophicus seryl-tRNA synthetase, MtSerRS, directly and stably interacts with arginyl-tRNA synthetase, MtArgRS, EC 6.1.1.19, two-hybrid system and surface plasmon resonance analysis, overview. The MtSerRS-MtArgRS complex also contains tRNAArg, consistent with the existence of a stable ribonucleoprotein complex active in aminoacylation. Deletion of the HTH motif, which contributes to the stability of SerRS dimers, significantly weakens the interaction between the two synthetases. Stimulation by MtArgRS peaks at 65°C ?
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg Methanothermobacter thermautotrophicus
-
 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 additional information Methanothermobacter thermautotrophicus arginyl-tRNA synthetase, MtArgRS, directly and stably interacts with seryl-tRNA synthetase, MtSerRS, EC 6.1.1.11, two-hybrid system and surface plasmon resonance analysis, overview. The MtSerRS-MtArgRS complex also contains tRNAArg, consistent with the existence of a stable ribonucleoprotein complex active in aminoacylation. Deletion of the HTH motif, which contributes to the stability of SerRS dimers, significantly weakens the interaction between the two synthetases. Stimulation by MtArgRS peaks at 65°C ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Methanothermobacter thermautotrophicus
-
-
-
6.1.1.19 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.11 GST-MtArgRS shows the ability to co-purify with His-tagged MtSerRS by nickel affinity chromatography, co-elution of the two enzymes during gel filtration Methanothermobacter thermautotrophicus
6.1.1.19 recombinant GST-tagged MtArgRS from Escherichia coli strain BL21(DE3) by glutathione affinity chromatograophy. GST-MtArgRS shows the ability to co-purify with His-tagged MtSerRS by nickel affinity chromatography, co-elution of the two enzymes during gel filtration Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 additional information seryl-tRNA synthetase, MtSerRS, directly and stably interacts with arginyl-tRNA synthetase, MtArgRS, EC 6.1.1.19, two-hybrid system and surface plasmon resonance analysis, overview. The MtSerRS-MtArgRS complex also contains tRNAArg, consistent with the existence of a stable ribonucleoprotein complex active in aminoacylation. Deletion of the HTH motif, which contributes to the stability of SerRS dimers, significantly weakens the interaction between the two synthetases. Stimulation by MtArgRS peaks at 65°C Methanothermobacter thermautotrophicus ?
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 additional information arginyl-tRNA synthetase, MtArgRS, directly and stably interacts with seryl-tRNA synthetase, MtSerRS, EC 6.1.1.11, two-hybrid system and surface plasmon resonance analysis, overview. The MtSerRS-MtArgRS complex also contains tRNAArg, consistent with the existence of a stable ribonucleoprotein complex active in aminoacylation. Deletion of the HTH motif, which contributes to the stability of SerRS dimers, significantly weakens the interaction between the two synthetases. Stimulation by MtArgRS peaks at 65°C Methanothermobacter thermautotrophicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.11 mtSerRS
-
 Methanothermobacter thermautotrophicus
6.1.1.11 SerRS
-
 Methanothermobacter thermautotrophicus
6.1.1.11 Seryl-tRNA synthetase
-
 Methanothermobacter thermautotrophicus
6.1.1.19 Arginyl-tRNA synthetase
-
 Methanothermobacter thermautotrophicus
6.1.1.19 ArgRS
-
 Methanothermobacter thermautotrophicus
6.1.1.19 MtArgRS
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.11 50
-
 assay at Methanothermobacter thermautotrophicus
6.1.1.19 50
-
 assay at Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.11 0.0073
-
 tRNASer pH 7.5, 50°C, recombinant MtSerRS Methanothermobacter thermautotrophicus
6.1.1.11 0.1
-
 tRNASer pH 7.5, 50°C, recombinant MtSerRS in presence of recombinant MtArgRS Methanothermobacter thermautotrophicus
6.1.1.19 0.026
-
 tRNAArg pH 7.5, 50°C, recombinant MtArgRS Methanothermobacter thermautotrophicus
6.1.1.19 0.034
-
 tRNAArg pH 7.5, 50°C, recombinant MtArgRS in presence of recombinant MtSerRS Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.11 7.5
-
 assay at Methanothermobacter thermautotrophicus
6.1.1.19 7.5
-
 assay at Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.11 ATP
-
 Methanothermobacter thermautotrophicus
6.1.1.19 ATP
-
 Methanothermobacter thermautotrophicus