BRENDA - Enzyme Database

Phosphatidate phosphatase activity plays key role in protection against fatty acid-induced toxicity in yeast

Fakas, S.; Qiu, Y.; Dixon, J.L.; Han, G.S.; Ruggles, K.V.; Garbarino, J.; Sturley, S.L.; Carman, G.M.; J. Biol. Chem. 286, 29074-29085 (2011)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
D398E
inactive
Saccharomyces cerevisiae
3.1.3.4
D400E
inactive
Saccharomyces cerevisiae
3.1.3.4
G80R
inactive
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
EDTA
complete inhibition at 2 mM
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
nucleus
-
Saccharomyces cerevisiae
5634
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
dependent on
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Saccharomyces cerevisiae
-
-
-
3.1.3.4
Saccharomyces cerevisiae W303-1A
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
715630
Saccharomyces cerevisiae
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
715630
Saccharomyces cerevisiae W303-1A
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
D398E
inactive
Saccharomyces cerevisiae
3.1.3.4
D400E
inactive
Saccharomyces cerevisiae
3.1.3.4
G80R
inactive
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
EDTA
complete inhibition at 2 mM
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
nucleus
-
Saccharomyces cerevisiae
5634
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
dependent on
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
715630
Saccharomyces cerevisiae
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
715630
Saccharomyces cerevisiae W303-1A
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
3.1.3.4
Saccharomyces cerevisiae
wild type cells supplemented with palmitoleic acid exhibit an induction in phosphatidate phosphatase activity
up
General Information
EC Number
General Information
Commentary
Organism
3.1.3.4
malfunction
cells lacking phosphatidate phosphatase are sensitive to exogenous fatty acids in the order of toxicity palmitoleic acid > oleic acid > palmitic acid
Saccharomyces cerevisiae
3.1.3.4
physiological function
phosphatidate phosphatase activity is essential in protecting cells from palmitoleic acid (fatty acid)-induced toxicity
Saccharomyces cerevisiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.3.4
malfunction
cells lacking phosphatidate phosphatase are sensitive to exogenous fatty acids in the order of toxicity palmitoleic acid > oleic acid > palmitic acid
Saccharomyces cerevisiae
3.1.3.4
physiological function
phosphatidate phosphatase activity is essential in protecting cells from palmitoleic acid (fatty acid)-induced toxicity
Saccharomyces cerevisiae
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.1.3.4
Saccharomyces cerevisiae
wild type cells supplemented with palmitoleic acid exhibit an induction in phosphatidate phosphatase activity
up