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Literature summary extracted from

  • Barta, M.L.; Skaff, D.A.; McWhorter, W.J.; Herdendorf, T.J.; Miziorko, H.M.; Geisbrecht, B.V.
    Crystal structures of Staphylococcus epidermidis mevalonate diphosphate decarboxylase bound to inhibitory analogs reveal new insight into substrate binding and catalysis (2011), J. Biol. Chem., 286, 23900-23910.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.33 expressed in Escherichia coli BL21(DE3) cells Staphylococcus epidermidis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.33 MDD bound to diphosphoglycolyl proline and 6-fluoromevalonate diphosphate, hanging drop vapor diffusion method, using 0.25 M sodium formate and 16% (w/v) PEG 3350, at 20°C Staphylococcus epidermidis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.33 S192A the mutation decreases kcat by about 1000fold Staphylococcus epidermidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.33 (R)-6-fluoromevalonate diphosphate
-
 Staphylococcus epidermidis
4.1.1.33 (R,S)-diphosphoglycolyl proline competitive inhibitor Staphylococcus epidermidis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.33 0.005
-
 (R,S)-5-diphosphomevalonate mutant enzyme S192A, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
4.1.1.33 0.0091
-
 (R,S)-5-diphosphomevalonate wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
4.1.1.33 0.027
-
 ATP wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
4.1.1.33 0.07
-
 ATP mutant enzyme S192A, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.33 Mg2+ required Staphylococcus epidermidis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.33 36000
-
 2 * 36000, gel filtration Staphylococcus epidermidis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.33 Staphylococcus epidermidis Q9FD73
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.33 Ni2+-NTA-Sepharose column chromatography and Resource Q anion-exchange chromatography Staphylococcus epidermidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.33 ATP + (R,S)-5-diphosphomevalonate
-
 Staphylococcus epidermidis ADP + phosphate + isopentenyl diphosphate + CO2
-
 ir

Subunits

EC Number Subunits Comment Organism
4.1.1.33 homodimer 2 * 36000, gel filtration Staphylococcus epidermidis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.33 MDD
-
 Staphylococcus epidermidis
4.1.1.33 mevalonate diphosphate decarboxylase
-
 Staphylococcus epidermidis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.33 0.0058
-
 (R,S)-5-diphosphomevalonate mutant enzyme S192A, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
4.1.1.33 5.9
-
 (R,S)-5-diphosphomevalonate wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.33 ATP
-
 Staphylococcus epidermidis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.33 0.000049
-
 (R)-6-fluoromevalonate diphosphate wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
4.1.1.33 0.0043
-
 (R,S)-diphosphoglycolyl proline wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis