EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.9 | expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni |
3.2.2.26 | expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni |
3.2.2.30 | expression in Escherichia coli as a His-tagged protein | Campylobacter jejuni |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.00093 | - |
5'-methylthioadenosine | in 50 mM potassium phosphate buffer, pH 7.0, at 25°C | Campylobacter jejuni | |
3.2.2.9 | 0.00103 | - |
6-amino-6-deoxyfutalosine | in 50 mM potassium phosphate buffer, pH 7.0, at 25°C | Campylobacter jejuni | |
3.2.2.26 | 0.00103 | - |
6-amino-6-deoxyfutalosine | at pH 7.0 and 25°C | Campylobacter jejuni | |
3.2.2.30 | 0.001 | - |
6-amino-6-deoxyfutalosine | pH 7, 25°C | Campylobacter jejuni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.9 | 5'-methylthioadenosine + H2O | Campylobacter jejuni | - |
5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.9 | 5'-methylthioadenosine + H2O | Campylobacter jejuni NCTC 11168 | - |
5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | Campylobacter jejuni | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | Campylobacter jejuni NCTC 11168 | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | Campylobacter jejuni | the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis | dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | Campylobacter jejuni NCTC 11168 | the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis | dehypoxanthine futalosine + adenine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.9 | Campylobacter jejuni | Q0PC20 | - |
- |
3.2.2.9 | Campylobacter jejuni NCTC 11168 | Q0PC20 | - |
- |
3.2.2.26 | Campylobacter jejuni | - |
- |
- |
3.2.2.26 | Campylobacter jejuni NCTC 11168 | - |
- |
- |
3.2.2.26 | Streptomyces coelicolor | Q9KXN0 | - |
- |
3.2.2.26 | Thermus thermophilus | Q5SKT7 | - |
- |
3.2.2.30 | Campylobacter jejuni | Q0PC20 | - |
- |
3.2.2.30 | Campylobacter jejuni NCTC 11168 | Q0PC20 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.9 | Ni-Sepharose column chromatography | Campylobacter jejuni |
3.2.2.26 | Sepharose column chromatography | Campylobacter jejuni |
3.2.2.30 | - |
Campylobacter jejuni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.9 | 5'-methylthioadenosine + H2O | - |
Campylobacter jejuni | 5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.9 | 5'-methylthioadenosine + H2O | - |
Campylobacter jejuni NCTC 11168 | 5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.9 | 6-amino-6-deoxyfutalosine + H2O | - |
Campylobacter jejuni | dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.9 | 6-amino-6-deoxyfutalosine + H2O | - |
Campylobacter jejuni NCTC 11168 | dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | - |
Campylobacter jejuni | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | - |
Campylobacter jejuni NCTC 11168 | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
3.2.2.26 | 6-amino-6-deoxyfutalosine + H2O | - |
Campylobacter jejuni | dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.26 | 6-amino-6-deoxyfutalosine + H2O | - |
Campylobacter jejuni NCTC 11168 | dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.26 | futalosine + H2O | - |
Thermus thermophilus | dehypoxanthine futalosine + hypoxanthine | - |
? | |
3.2.2.26 | futalosine + H2O | - |
Streptomyces coelicolor | dehypoxanthine futalosine + hypoxanthine | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis | Campylobacter jejuni | dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate. The activity with futalosine ist 2% compared to the activity with 6-amino-6-deoxyfutalosine. The enzyme also has the activity of EC 3.2.2.9, adenosylhomocysteine nucleosidase | Campylobacter jejuni | dehypoxanthine futalosine + adenine | dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate | ? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis | Campylobacter jejuni NCTC 11168 | dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate. The activity with futalosine ist 2% compared to the activity with 6-amino-6-deoxyfutalosine. The enzyme also has the activity of EC 3.2.2.9, adenosylhomocysteine nucleosidase | Campylobacter jejuni NCTC 11168 | dehypoxanthine futalosine + adenine | dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.9 | 5'-methylthioadenosine nucleosidase | - |
Campylobacter jejuni |
3.2.2.9 | Cj0117 | - |
Campylobacter jejuni |
3.2.2.9 | MTAN | - |
Campylobacter jejuni |
3.2.2.26 | 5'-methylthioadenosine nucleosidase | - |
Campylobacter jejuni |
3.2.2.26 | futalosine nucleosidase | - |
Thermus thermophilus |
3.2.2.26 | futalosine nucleosidase | - |
Streptomyces coelicolor |
3.2.2.26 | MqnB | - |
Thermus thermophilus |
3.2.2.26 | MqnB | - |
Streptomyces coelicolor |
3.2.2.26 | MTAN | - |
Campylobacter jejuni |
3.2.2.30 | AFL nucleosidase | - |
Campylobacter jejuni |
3.2.2.30 | aminofutalosine nucleosidase | - |
Campylobacter jejuni |
3.2.2.30 | Cj0117 | - |
Campylobacter jejuni |
3.2.2.30 | methylthioadenosine nucleosidase | - |
Campylobacter jejuni |
3.2.2.30 | MqnB | - |
Campylobacter jejuni |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.30 | 25 | - |
assay at | Campylobacter jejuni |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.53 | - |
6-amino-6-deoxyfutalosine | in 50 mM potassium phosphate buffer, pH 7.0, at 25°C | Campylobacter jejuni | |
3.2.2.9 | 2.7 | - |
5'-methylthioadenosine | in 50 mM potassium phosphate buffer, pH 7.0, at 25°C | Campylobacter jejuni | |
3.2.2.26 | 0.53 | - |
6-amino-6-deoxyfutalosine | at pH 7.0 and 25°C | Campylobacter jejuni | |
3.2.2.30 | 0.53 | - |
6-amino-6-deoxyfutalosine | pH 7, 25°C | Campylobacter jejuni |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.30 | 7 | - |
assay at | Campylobacter jejuni |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.2.9 | physiological function | the enzyme plays key roles in S-adenosylmethionine recycling, the biosynthesis of autoinducer molecules, and the biosynthesis of menaquinone | Campylobacter jejuni |
3.2.2.30 | physiological function | the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis | Campylobacter jejuni |