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Literature summary extracted from

  • Li, X.; Apel, D.; Gaynor, E.C.; Tanner, M.E.
    5'-methylthioadenosine nucleosidase is implicated in playing a key role in a modified futalosine pathway for menaquinone biosynthesis in Campylobacter jejuni (2011), J. Biol. Chem., 286, 19392-19398.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.9 expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni
3.2.2.26 expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni
3.2.2.30 expression in Escherichia coli as a His-tagged protein Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.9 0.00093
-
 5'-methylthioadenosine in 50 mM potassium phosphate buffer, pH 7.0, at 25°C Campylobacter jejuni
3.2.2.9 0.00103
-
 6-amino-6-deoxyfutalosine in 50 mM potassium phosphate buffer, pH 7.0, at 25°C Campylobacter jejuni
3.2.2.26 0.00103
-
 6-amino-6-deoxyfutalosine at pH 7.0 and 25°C Campylobacter jejuni
3.2.2.30 0.001
-
 6-amino-6-deoxyfutalosine pH 7, 25°C Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.9 5'-methylthioadenosine + H2O Campylobacter jejuni
-
 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Campylobacter jejuni NCTC 11168
-
 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Campylobacter jejuni
-
 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Campylobacter jejuni NCTC 11168
-
 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O Campylobacter jejuni the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis dehypoxanthine futalosine + adenine
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O Campylobacter jejuni NCTC 11168 the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis dehypoxanthine futalosine + adenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.9 Campylobacter jejuni Q0PC20
-
-
3.2.2.9 Campylobacter jejuni NCTC 11168 Q0PC20
-
-
3.2.2.26 Campylobacter jejuni
-
-
-
3.2.2.26 Campylobacter jejuni NCTC 11168
-
-
-
3.2.2.26 Streptomyces coelicolor Q9KXN0
-
-
3.2.2.26 Thermus thermophilus Q5SKT7
-
-
3.2.2.30 Campylobacter jejuni Q0PC20
-
-
3.2.2.30 Campylobacter jejuni NCTC 11168 Q0PC20
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.9 Ni-Sepharose column chromatography Campylobacter jejuni
3.2.2.26 Sepharose column chromatography Campylobacter jejuni
3.2.2.30
-
 Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.9 5'-methylthioadenosine + H2O
-
 Campylobacter jejuni 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Campylobacter jejuni NCTC 11168 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 6-amino-6-deoxyfutalosine + H2O
-
 Campylobacter jejuni dehypoxanthine futalosine + adenine
-
 ?
3.2.2.9 6-amino-6-deoxyfutalosine + H2O
-
 Campylobacter jejuni NCTC 11168 dehypoxanthine futalosine + adenine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Campylobacter jejuni S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Campylobacter jejuni NCTC 11168 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.26 6-amino-6-deoxyfutalosine + H2O
-
 Campylobacter jejuni dehypoxanthine futalosine + adenine
-
 ?
3.2.2.26 6-amino-6-deoxyfutalosine + H2O
-
 Campylobacter jejuni NCTC 11168 dehypoxanthine futalosine + adenine
-
 ?
3.2.2.26 futalosine + H2O
-
 Thermus thermophilus dehypoxanthine futalosine + hypoxanthine
-
 ?
3.2.2.26 futalosine + H2O
-
 Streptomyces coelicolor dehypoxanthine futalosine + hypoxanthine
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis Campylobacter jejuni dehypoxanthine futalosine + adenine
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate. The activity with futalosine ist 2% compared to the activity with 6-amino-6-deoxyfutalosine. The enzyme also has the activity of EC 3.2.2.9, adenosylhomocysteine nucleosidase Campylobacter jejuni dehypoxanthine futalosine + adenine dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis Campylobacter jejuni NCTC 11168 dehypoxanthine futalosine + adenine
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate. The activity with futalosine ist 2% compared to the activity with 6-amino-6-deoxyfutalosine. The enzyme also has the activity of EC 3.2.2.9, adenosylhomocysteine nucleosidase Campylobacter jejuni NCTC 11168 dehypoxanthine futalosine + adenine dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.9 5'-methylthioadenosine nucleosidase
-
 Campylobacter jejuni
3.2.2.9 Cj0117
-
 Campylobacter jejuni
3.2.2.9 MTAN
-
 Campylobacter jejuni
3.2.2.26 5'-methylthioadenosine nucleosidase
-
 Campylobacter jejuni
3.2.2.26 futalosine nucleosidase
-
 Thermus thermophilus
3.2.2.26 futalosine nucleosidase
-
 Streptomyces coelicolor
3.2.2.26 MqnB
-
 Thermus thermophilus
3.2.2.26 MqnB
-
 Streptomyces coelicolor
3.2.2.26 MTAN
-
 Campylobacter jejuni
3.2.2.30 AFL nucleosidase
-
 Campylobacter jejuni
3.2.2.30 aminofutalosine nucleosidase
-
 Campylobacter jejuni
3.2.2.30 Cj0117
-
 Campylobacter jejuni
3.2.2.30 methylthioadenosine nucleosidase
-
 Campylobacter jejuni
3.2.2.30 MqnB
-
 Campylobacter jejuni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.30 25
-
 assay at Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.9 0.53
-
 6-amino-6-deoxyfutalosine in 50 mM potassium phosphate buffer, pH 7.0, at 25°C Campylobacter jejuni
3.2.2.9 2.7
-
 5'-methylthioadenosine in 50 mM potassium phosphate buffer, pH 7.0, at 25°C Campylobacter jejuni
3.2.2.26 0.53
-
 6-amino-6-deoxyfutalosine at pH 7.0 and 25°C Campylobacter jejuni
3.2.2.30 0.53
-
 6-amino-6-deoxyfutalosine pH 7, 25°C Campylobacter jejuni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.30 7
-
 assay at Campylobacter jejuni

General Information

EC Number General Information Comment Organism
3.2.2.9 physiological function the enzyme plays key roles in S-adenosylmethionine recycling, the biosynthesis of autoinducer molecules, and the biosynthesis of menaquinone Campylobacter jejuni
3.2.2.30 physiological function the enzyme is part of a modified futalosine pathway for menaquinone biosynthesis Campylobacter jejuni