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Literature summary extracted from

  • Franklin, N.E.; Taylor, G.S.; Vacratsis, P.O.
    Endosomal targeting of the phosphoinositide 3-phosphatase MTMR2 is regulated by an N-terminal phosphorylation site (2011), J. Biol. Chem., 286, 15841-15853.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.64 overexpression of wild-type MTMR2 in HeLa cells predominantly located to the cytosolic/perinuclear region, recombinant expression of FLAG-tagged and of His-tagged wild-type and mutant enzymes in Escherichia coli lysates Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.64 C417S site-directed mutagenesis, a catalytically inactive MTMR2 variant Homo sapiens
3.1.3.64 S58A site-directed mutagenesis, a phosphorylation-mimetic mutant, which retains full catalytic activity toward PI(3)P and PI(3,5)P2 as compared to wild-type MTMR2. The majority of the MTMR2 phosphorylation-deficient S58A mutant is localized to distinct punctate structures, indicative of endosomal localization, in contrast to the wild-type enzyme. The level of ERK1/2 phosphorylation/activation is also2-3fold higher in cells expressing MTMR2S58A in comparison to control cells Homo sapiens
3.1.3.64 S58A/C417S site-directed mutagenesis, a catalytically inactive MTMR2 variant, which shows strong co-localization with markers for early endosomes including the PI(3)P marker generated from EEA1 and Rab5 Homo sapiens
3.1.3.64 S58E site-directed mutagenesis, a phosphorylation-deficient mutant, which retains full catalytic activity toward PI(3)P and PI(3,5)P2 as compared to wild-type MTMR2. The mutant enzyme displays a similar localization pattern as wild-type in the cytoplasm Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.64 Homo sapiens
-
 gene mtmr2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.64 recombinant FLAG-tagged and His-tagged wild-type and mutant enzymes from Escherichia coli lysates Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
3.1.3.64 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.64 MTMR2
-
 Homo sapiens
3.1.3.64 phosphoinositide 3-phosphatase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.64 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.64 7
-
 assay at Homo sapiens