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Literature summary extracted from

  • Gregg, K.J.; Zandberg, W.F.; Hehemann, J.H.; Whitworth, G.E.; Deng, L.; Vocadlo, D.J.; Boraston, A.B.
    Analysis of a new family of widely distributed metal-independent alpha-mannosidases provides unique insight into the processing of N-linked glycans (2011), J. Biol. Chem., 286, 15586-15596.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.24 expressed in Escherichia coli as a His-tagged fusion protein Streptococcus pneumoniae
3.2.1.24 expressed in Escherichia coli as a His-tagged fusion protein Clostridium perfringens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.24 X-ray crystal structures obtained in apo-, inhibitor-bound, and substrate-bound forms provide both mechanistic and molecular insight into how the proteins, which adopts an (alpha/alpha)6fold, recognizes and hydrolyzes the alpha1,6-mannosidic bond by an inverting, metal-independent catalytic mechanism Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Clostridium perfringens
-
-
-
3.2.1.24 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 using Ni-NTA chromatography Streptococcus pneumoniae
3.2.1.24 using Ni-NTA chromatography Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
-
 Streptococcus pneumoniae 2,4-dinitrophenol + alpha-D-mannopyranose
-
 ?
3.2.1.24 2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
-
 Clostridium perfringens 2,4-dinitrophenol + alpha-D-mannopyranose
-
 ?
3.2.1.24 additional information enzyme processes alpha1,6-mannobiose into the product mannose. No activity on alpha1,2-mannobiose, alpha1,3-mannobiose or alpha(1,3)/(1,6)-mannotriose Streptococcus pneumoniae ?
-
 ?
3.2.1.24 additional information enzyme processes alpha1,6-mannobiose into the product mannose. No activity on alpha1,2-mannobiose, alpha1,3-mannobiose or alpha(1,3)/(1,6)-mannotriose Clostridium perfringens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.24 CpGH125
-
 Clostridium perfringens
3.2.1.24 SpGH125
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.24 25
-
 assay with 2,4-dinitrophenyl alpha-D-mannopyranoside Streptococcus pneumoniae
3.2.1.24 25
-
 assay with 2,4-dinitrophenyl alpha-D-mannopyranoside Clostridium perfringens
3.2.1.24 37
-
 assay at Streptococcus pneumoniae
3.2.1.24 37
-
 assay at Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 7.4
-
 assay at Streptococcus pneumoniae
3.2.1.24 7.4
-
 assay at Clostridium perfringens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.24 0.00216
-
 2,4-dinitrophenyl alpha-D-mannopyranoside pH 7.4, 25°C Streptococcus pneumoniae
3.2.1.24 0.00966
-
 2,4-dinitrophenyl alpha-D-mannopyranoside pH 7.4, 25°C Clostridium perfringens