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Literature summary extracted from

  • Bacik, J.P.; Whitworth, G.E.; Stubbs, K.A.; Yadav, A.K.; Martin, D.R.; Bailey-Elkin, B.A.; Vocadlo, D.J.; Mark, B.L.
    Molecular basis of 1,6-anhydro bond cleavage and phosphoryl transfer by Pseudomonas aeruginosa 1,6-anhydro-N-acetylmuramic acid kinase (2011), J. Biol. Chem., 286, 12283-12291.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.170 expressed in Escherichia coli BL21(DE3) Gold cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.170 AnmK in complex with 1,6-anhydro-N-acetylmuramic acid and ADP, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 4000, 0.2 M (NH4)2SO4, and 0.1 M tri-sodium citrate (pH 5.6) Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.170 0.2
-
 1,6-anhydro-N-acetyl-beta-muramate in 50 mM HEPES, pH 7.5, 10 mM MgCl2, and 50 mM KCl, at 37°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.170 Mg2+ required Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.170 Pseudomonas aeruginosa Q9I5Q5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.170 Ni-NTA-agarose column chromatography and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.170 ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O
-
 Pseudomonas aeruginosa ADP + N-acetyl-beta-muramate 6-phosphate + H+
-
 ?
2.7.1.170 additional information AnmK is active only against 1,6-anhydro-N-acetyl-beta-muramate and not against 1,6-anhydro-N-acetylmuramyl peptides Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.170 homodimer x-ray crystallography Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.7.1.170 1,6-anhydro-N-acetylmuramic acid kinase
-
 Pseudomonas aeruginosa
2.7.1.170 AnmK
-
 Pseudomonas aeruginosa