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Literature summary extracted from

  • Jeanguenin, L.; Lara-Nunez, A.; Pribat, A.; Mageroy, M.H.; Gregory, J.F.; Rice, K.C.; de Crecy-Lagard, V.; Hanson, A.D.
    Moonlighting glutamate formiminotransferases can functionally replace 5-formyltetrahydrofolate cycloligase (2010), J. Biol. Chem., 285, 41557-41566.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.3.3.2 analysis development of a functional complementation assay for 5-CHO-THF metabolism in Escherichia coli, based on deleting the gene encoding 5-FCL. The deletion mutant accumulates 5-formyltetrahydrofolate and,with glycine as sole nitrogen source, shows a growth defect, both phenotypes are complemented by bacterial or archaeal genes encoding glutamate formiminotransferase. Glutamate formiminotransferases functionally replace 5-formyltetrahydrofolate cyclo-ligases in certain prokaryotes Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.5 glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-CHO-THF cycloligase deletion mutant with strain MG1655 background Streptococcus pyogenes
2.1.2.5 glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background Thermoplasma acidophilum
2.1.2.5 glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background Picrophilus torridus
2.1.2.5 glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-N-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background Candidatus Koribacter versatilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.5 0.0004
-
 5-formimidoyltetrahydrofolate pH 7.3, 30°C Picrophilus torridus
2.1.2.5 0.0007
-
 N-formimidoyl-L-Glu-L-Glu-L-Glu pH 7.3, 30°C Picrophilus torridus
2.1.2.5 0.0018
-
 5-formimidoyltetrahydrofolate pH 7.3, 30°C Candidatus Koribacter versatilis
2.1.2.5 0.0037
-
 5-formimidoyltetrahydrofolate pH 7.3, 30°C Thermoplasma acidophilum
2.1.2.5 0.0038
-
 N-formimidoyl-L-Glu-L-Glu-L-Glu pH 7.3, 30°C Thermoplasma acidophilum
2.1.2.5 0.0048
-
 N-formimidoyl-L-Glu-L-Glu-L-Glu pH 7.3, 30°C Streptococcus pyogenes
2.1.2.5 0.0054
-
 5-formimidoyltetrahydrofolate pH 7.3, 30°C Streptococcus pyogenes
2.1.2.5 0.0058
-
 N-formimidoyl-L-Glu-L-Glu-L-Glu pH 7.3, 30°C Candidatus Koribacter versatilis
2.1.2.5 0.034
-
 L-glutamate pH 7.3, 30°C Picrophilus torridus
2.1.2.5 0.113
-
 L-glutamate pH 7.3, 30°C Thermoplasma acidophilum
2.1.2.5 0.206
-
 L-glutamate pH 7.3, 30°C Candidatus Koribacter versatilis
2.1.2.5 1.03
-
 L-glutamate pH 7.3, 30°C Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate Thermoplasma acidophilum
-
 tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate Streptococcus pyogenes
-
 tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate Picrophilus torridus
-
 tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate Candidatus Koribacter versatilis
-
 tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate Candidatus Koribacter versatilis Ellin345
-
 tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate Streptococcus pyogenes M1
-
 tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.5 Candidatus Koribacter versatilis
-
-
-
2.1.2.5 Candidatus Koribacter versatilis Ellin345
-
-
-
2.1.2.5 no activity in Herpetosiphon aurantiacus
-
 the gene encoding glycine formimidoyltransferase is an inactive pseudogene, the histidine utilization hut operon in Herpetosiphon aurantiacus is entirely broken up, suggesting that this pathway no longer functions
-
2.1.2.5 Picrophilus torridus
-
-
-
2.1.2.5 Streptococcus pyogenes
-
-
-
2.1.2.5 Streptococcus pyogenes M1
-
-
-
2.1.2.5 Thermoplasma acidophilum
-
-
-
6.3.3.2 Escherichia coli A0A061KWR9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.5 recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography Thermoplasma acidophilum
2.1.2.5 recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography Streptococcus pyogenes
2.1.2.5 recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography Picrophilus torridus
2.1.2.5 recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography Candidatus Koribacter versatilis

Storage Stability

EC Number Storage Stability Organism
2.1.2.5 -80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, loss of 80% activity after 8 weeks Candidatus Koribacter versatilis
2.1.2.5 -80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks Thermoplasma acidophilum
2.1.2.5 -80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks Streptococcus pyogenes
2.1.2.5 -80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks Picrophilus torridus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate
-
 Thermoplasma acidophilum tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate
-
 Streptococcus pyogenes tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate
-
 Picrophilus torridus tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate
-
 Candidatus Koribacter versatilis tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate
-
 Candidatus Koribacter versatilis Ellin345 tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate
-
 Streptococcus pyogenes M1 tetrahydrofolate + N-formimidoyl-L-glutamate
-
 ?
2.1.2.5 N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
 Thermoplasma acidophilum N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
 ?
2.1.2.5 N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
 Streptococcus pyogenes N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
 ?
2.1.2.5 N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
 Picrophilus torridus N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
 ?
2.1.2.5 N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
 Candidatus Koribacter versatilis N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
 ?
2.1.2.5 N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
 Candidatus Koribacter versatilis Ellin345 N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
 ?
2.1.2.5 N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate
-
 Streptococcus pyogenes M1 N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.5 glutamate formiminotransferase
-
 Thermoplasma acidophilum
2.1.2.5 glutamate formiminotransferase
-
 Streptococcus pyogenes
2.1.2.5 glutamate formiminotransferase
-
 Picrophilus torridus
2.1.2.5 glutamate formiminotransferase
-
 Candidatus Koribacter versatilis
6.3.3.2 ygfA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.5 30
-
 assay at Thermoplasma acidophilum
2.1.2.5 30
-
 assay at Streptococcus pyogenes
2.1.2.5 30
-
 assay at Picrophilus torridus
2.1.2.5 30
-
 assay at Candidatus Koribacter versatilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.5 0.0022
-
 L-glutamate pH 7.3, 30°C Streptococcus pyogenes
2.1.2.5 0.0022
-
 5-formimidoyltetrahydrofolate pH 7.3, 30°C Streptococcus pyogenes
2.1.2.5 0.0045
-
 L-glutamate pH 7.3, 30°C Thermoplasma acidophilum
2.1.2.5 0.0045
-
 5-formimidoyltetrahydrofolate pH 7.3, 30°C Thermoplasma acidophilum
2.1.2.5 0.0092
-
 L-glutamate pH 7.3, 30°C Candidatus Koribacter versatilis
2.1.2.5 0.0092
-
 5-formimidoyltetrahydrofolate pH 7.3, 30°C Candidatus Koribacter versatilis
2.1.2.5 0.0193
-
 L-glutamate pH 7.3, 30°C Picrophilus torridus
2.1.2.5 0.0193
-
 5-formimidoyltetrahydrofolate pH 7.3, 30°C Picrophilus torridus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.5 7.3
-
 assay at Thermoplasma acidophilum
2.1.2.5 7.3
-
 assay at Streptococcus pyogenes
2.1.2.5 7.3
-
 assay at Picrophilus torridus
2.1.2.5 7.3
-
 assay at Candidatus Koribacter versatilis

General Information

EC Number General Information Comment Organism
2.1.2.5 metabolism glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview Thermoplasma acidophilum
2.1.2.5 metabolism glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview Streptococcus pyogenes
2.1.2.5 metabolism glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview Picrophilus torridus
2.1.2.5 metabolism glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview Candidatus Koribacter versatilis
2.1.2.5 physiological function utilization of supplied 5-formyltetrahydrofolate by Streptococcus pyogenes requires expression of the native glutamate formiminotransferase Streptococcus pyogenes