Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chow, J.Y.; Xue, B.; Lee, K.H.; Tung, A.; Wu, L.; Robinson, R.C.; Yew, W.S.
    Directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily (2010), J. Biol. Chem., 285, 40911-40920.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 expressed in Escherichia coli BL21(DE3) cells Geobacillus kaustophilus
3.1.1.81 expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Geobacillus kaustophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.81 D266N mutant, sitting drop vapor diffusion method, using 100 mM Tris, pH 7.5, 20% (w/v) PEG 4000, and 1.0 mM ZnCl2 Geobacillus kaustophilus
3.1.1.81 purified mutant D266N in complex with N-butyryl-L-homoserine lactone, sitting-drop vapor diffusion method, equal volumes of 45 mg/ml protein in 100 mM NaCl, 20 mM Tris-HCl, pH 8.0, and 1 mM N-butyryl-L-homoserine lactone, are mixed with precipitation solution containing 100 mM Tris, pH 7.5, 20% w/v PEG 4000, and 1.0 mM ZnCl2, 3 days, 20°C, X-ray diffraction structure determination and analysis at 1.6 A resolution Geobacillus kaustophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.81 D266N catalytically inactive mutant Geobacillus kaustophilus
3.1.1.81 D266N inactive Geobacillus kaustophilus
3.1.1.81 E101G/R230C the mutant has an increased value of kcat/Km of 72fold toward 3-oxo-N-dodecanoyl-L-homoserine lactone Geobacillus kaustophilus
3.1.1.81 E101G/R230C the mutant shows increased catalytic efficiency compared to the wild type enzyme Geobacillus kaustophilus
3.1.1.81 E101K/R230P the mutant shows decreased catalytic efficiency toward 3-oxo-N-octanoyl-L-homoserine lactone and no activioty towards 3-oxo-N-hexanoyl-L-homoserine lactone compared to the wild type enzyme Geobacillus kaustophilus
3.1.1.81 E101N inactive Geobacillus kaustophilus
3.1.1.81 E101N/R230I the mutant has an increased value of kcat/Km of 72fold toward 3-oxo-N-dodecanoyl-L-homoserine lactone Geobacillus kaustophilus
3.1.1.81 E101N/R230I the mutant has an 72fold increased kcat/Km value toward 3-oxo-N-dodecanoyl-L-homoserine lactone compared to the wild type enzyme and also exhibits lactonase activity toward N-butyryl-L-homoserine lactone Geobacillus kaustophilus
3.1.1.81 E101V/R230T the mutant shows increased catalytic efficiency compared to the wild type enzyme Geobacillus kaustophilus
3.1.1.81 R230D inactive Geobacillus kaustophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.81 0.0024
-
 gamma-nonalactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.024
-
 gamma-nonalactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.035
-
 delta-nonalactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.035
-
 delta-nonalactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.095
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.095
-
 N-octanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.13
-
 N-decanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.13
-
 N-decanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.13
-
 N-decanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.13
-
 N-decanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.39
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.39
-
 N-octanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.46
-
 gamma-nonalactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.46
-
 gamma-nonalactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.48
-
 N-3-oxo-dodecanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.48
-
 N-(3-oxododecanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.69
-
 paraoxon wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.74
-
 N-3-oxo-dodecanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.74
-
 N-(3-oxododecanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.1
-
 delta-nonalactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 1.1
-
 gamma-nonalactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.1
-
 delta-nonalactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.2
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 1.2
-
 N-octanoyl-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 2.4
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 2.4
-
 N-hexanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 2.6
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 2.6
-
 N-hexanoyl-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 2.9
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 2.9
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 2.9
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 3.7
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 3.7
-
 N-hexanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 3.8
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 3.8
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 5.9
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 5.9
-
 N-(3-oxooctanoyl)-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 9
-
 N-3-oxo-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 9
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 9
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 26
-
 N-3-oxo-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 26
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.81 Fe2+ bound to wild-type as well as mutant enzymes Geobacillus kaustophilus
3.1.1.81 Fe2+ contains iron Geobacillus kaustophilus
3.1.1.81 Zn2+ bound to wild-type as well as mutant enzymes Geobacillus kaustophilus
3.1.1.81 Zn2+ contains zinc, required for activity Geobacillus kaustophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Geobacillus kaustophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81 Ni2+-Sepharose column chromatography and Q-Sepharose column chromatography Geobacillus kaustophilus
3.1.1.81 recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin, followed by anion exchange chromatography, to homogeneity Geobacillus kaustophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 delta-nonalactone + H2O
-
 Geobacillus kaustophilus 5-hydroxynonanoate
-
 ?
3.1.1.81 delta-nonalactone + H2O high activity Geobacillus kaustophilus 5-hydroxynonanoate
-
 ?
3.1.1.81 gamma-nonalactone + H2O
-
 Geobacillus kaustophilus 4-hydroxynonanoate
-
 ?
3.1.1.81 gamma-nonalactone + H2O high activity Geobacillus kaustophilus 4-hydroxynonanoate
-
 ?
3.1.1.81 additional information the wild-type enzyme does also show low hydrolysis activity with paraoxon, while its mutants do not Geobacillus kaustophilus ?
-
 ?
3.1.1.81 additional information no activity towards N-butyryl-L-homoserine lactone, N-decanoyl-L-homoserine lactone, and N-(3-oxohexanoyl)-L-homoserine lactone Geobacillus kaustophilus ?
-
 ?
3.1.1.81 N-(3-oxododecanoyl)-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-(3-oxododecanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxohexanoyl)-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-(3-oxohexanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxooctanoyl)-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-(3-oxooctanoyl)-L-homoserine
-
 ?
3.1.1.81 N-3-oxo-dodecanoyl-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-3-oxo-dodecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxo-hexanoyl-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-3-oxo-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxo-octanoyl-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-3-oxo-octanoyl-L-homoserine
-
 ?
3.1.1.81 N-butyryl-L-homoserine lactone + H2O substrate of mutant E101N/R230I, not of the wild-type enzyme Geobacillus kaustophilus N-butyryl-L-homoserine
-
 ?
3.1.1.81 N-butyryl-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-butyryl-L-homoserine
-
 ?
3.1.1.81 N-butyryl-L-homoserine lactone + H2O substrate for mutant enzymes E101G/R230C and E101N/R230I Geobacillus kaustophilus N-butyryl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-DL-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-DL-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-octanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Geobacillus kaustophilus N-octanoyl-L-homoserine
-
 ?
3.1.1.81 paraoxon + H2O low activity Geobacillus kaustophilus diethylphosphate + 4-nitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 GKL
-
 Geobacillus kaustophilus
3.1.1.81 additional information the enzyme belongs to the phosphotriesterase-like lactonase group of enzymes within the amidohydrolase superfamily Geobacillus kaustophilus
3.1.1.81 N-acylhomoserine lactonase
-
 Geobacillus kaustophilus
3.1.1.81 quorum-quenching lactonase
-
 Geobacillus kaustophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.81 37
-
 assay at Geobacillus kaustophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.81 60
-
 the wild type enzyme retains its catalytic activity even after exposure to 60°C for 72 h Geobacillus kaustophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.81 0.0031
-
 paraoxon wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.009
-
 N-butyryl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.009
-
 N-butyryl-L-homoserine lactone kcat below 0.009 s-1, mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.011
-
 N-butyryl-L-homoserine lactone kcat below 0.011 s-1, mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.17
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.17
-
 N-hexanoyl-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.2
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.2
-
 N-hexanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.25
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.25
-
 N-hexanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.29
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.29
-
 N-(3-oxooctanoyl)-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.3
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.3
-
 N-octanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.4
-
 N-3-oxo-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.4
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.4
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.43
-
 N-decanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.43
-
 N-decanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.52
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.52
-
 N-octanoyl-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.91
-
 N-3-oxo-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.91
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.1
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 1.1
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.4
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 1.4
-
 N-octanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.6
-
 delta-nonalactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 1.6
-
 delta-nonalactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.7
-
 N-decanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 1.7
-
 N-decanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.8
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 1.8
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.8
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 2
-
 N-3-oxo-dodecanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 2
-
 gamma-nonalactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 2
-
 N-(3-oxododecanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 2
-
 gamma-nonalactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 2.9
-
 gamma-nonalactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 2.9
-
 gamma-nonalactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 6.9
-
 N-3-oxo-dodecanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 6.9
-
 N-(3-oxododecanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 22
-
 gamma-nonalactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 29
-
 delta-nonalactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 29
-
 delta-nonalactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.81 8.3
-
 assay at Geobacillus kaustophilus

General Information

EC Number General Information Comment Organism
3.1.1.81 evolution directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily Geobacillus kaustophilus
3.1.1.81 additional information a charge transfer complex involving the beta-cation and Tyr99 within the enzyme active site determines the coloration of the organism. Modulation of the charge transfer complex alters the lactonase activity of the mutant enzymes and is reflected in enzyme coloration changes Geobacillus kaustophilus
3.1.1.81 physiological function the enzyme hydrolyze N-acylhomoserine lactones that are involved in virulence pathways of quorum-sensing pathogenic bacteria Geobacillus kaustophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.81 0.0045
-
 N-3-oxo-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.0045
-
 N-(3-oxohexanoyl)-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.0045
-
 paraoxon wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.017
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101V/R230T, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.021
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101K/R230P, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.035
-
 N-3-oxo-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.035
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.045
-
 N-3-oxo-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.045
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.045
-
 N-(3-oxohexanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.05
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.05
-
 N-(3-oxooctanoyl)-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.066
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.066
-
 N-hexanoyl-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.068
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.068
-
 N-hexanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.082
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.082
-
 N-hexanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.11
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101V/R230T, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.13
-
 N-3-oxo-dodecanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.13
-
 N-(3-oxododecanoyl)-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.28
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 0.28
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.3
-
 N-decanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.3
-
 N-decanoyl-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.43
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 0.43
-
 N-octanoyl-L-homoserine lactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.64
-
 N-3-oxo-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 0.64
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 0.64
-
 N-(3-oxooctanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 1.4
-
 delta-nonalactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 1.4
-
 delta-nonalactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 3.2
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 3.2
-
 N-octanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 3.3
-
 N-decanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 3.3
-
 N-decanoyl-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 3.7
-
 N-octanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 3.7
-
 N-octanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 3.9
-
 delta-nonalactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 3.9
-
 delta-nonalactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 4.2
-
 N-3-oxo-dodecanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 4.2
-
 N-(3-oxododecanoyl)-L-homoserine lactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 4.3
-
 gamma-nonalactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 4.3
-
 gamma-nonalactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 9.3
-
 N-3-oxo-dodecanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 9.3
-
 N-(3-oxododecanoyl)-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 13
-
 N-decanoyl-L-homoserine lactone pH 8.3, 37°C, recombinant mutant E101N/R230I Geobacillus kaustophilus
3.1.1.81 13
-
 N-decanoyl-L-homoserine lactone mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 21
-
 gamma-nonalactone pH 8.3, 37°C, recombinant mutant E101G/R230C Geobacillus kaustophilus
3.1.1.81 21
-
 gamma-nonalactone mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 120
-
 gamma-nonalactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 120
-
 gamma-nonalactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus
3.1.1.81 840
-
 delta-nonalactone pH 8.3, 37°C, recombinant wild-type enzyme Geobacillus kaustophilus
3.1.1.81 840
-
 delta-nonalactone wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C Geobacillus kaustophilus