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Literature summary extracted from

  • Rinaldo-Matthis, A.; Wetterholm, A.; Martinez Molina, D.; Holm, J.; Niegowski, D.; Ohlson, E.; Nordlund, P.; Morgenstern, R.; Haeggstroem, J.Z.
    Arginine 104 is a key catalytic residue in leukotriene C4 synthase (2010), J. Biol. Chem., 285, 40771-40776.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.20 expression in Pichia pastoris Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.20 the interaction of residue R104 with the thiol group of GSH reduces its pKa to allow formation of a thiolate anion and subsequent nucleophilic attack at C6 of LTA4.Crystal structure of mutant R31Q at 2.1 A reveals a Q31 side chain pointing away from the active site Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.20 R104A 5.7% of wild-type activity Homo sapiens
4.4.1.20 R104K 0.1% of wild-type activity Homo sapiens
4.4.1.20 R104S 4.6% of wild-type activity Homo sapiens
4.4.1.20 R104T 1.1% of wild-type activity Homo sapiens
4.4.1.20 R31A 12% of wild-type activity Homo sapiens
4.4.1.20 R31Q 30% of wild-type activity Homo sapiens
4.4.1.20 R51A 57% of wild-type activity Homo sapiens
4.4.1.20 R51Q 29% of wild-type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.20 0.05
-
 leukotriene A4 mutant R31A, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 0.07
-
 leukotriene A4 mutant R31Q, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 0.1
-
 leukotriene A4 mutant R104S, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 0.3
-
 leukotriene A4 wild-type, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 0.5
-
 leukotriene A4 mutant R104A, 22°C, pH not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.20 Homo sapiens Q16873
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.20 0.044
-
 mutant R104K, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 0.4
-
 mutant R104T, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 1.6
-
 mutant R104S, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 2
-
 mutant R104A, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 4.3
-
 mutant R31A, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 10
-
 mutant R51Q, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 10.5
-
 mutant R31Q, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 20
-
 mutant R51A, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 35
-
 wild-type, 22°C, pH not specified in the publication Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.20 leukotriene A4 + glutathione
-
 Homo sapiens leukotriene C4
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.20 0.5
-
 leukotriene A4 mutant R104S, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 0.66
-
 leukotriene A4 mutant R104A, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 1.4
-
 leukotriene A4 mutant R31A, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 1.8
-
 leukotriene A4 mutant R31Q, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 11.7
-
 leukotriene A4 wild-type, 22°C, pH not specified in the publication Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.20 1.3
-
 leukotriene A4 mutant R104A, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 5
-
 leukotriene A4 mutant R104S, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 26
-
 leukotriene A4 mutant R31Q, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 28
-
 leukotriene A4 mutant R31A, 22°C, pH not specified in the publication Homo sapiens
4.4.1.20 39
-
 leukotriene A4 wild-type, 22°C, pH not specified in the publication Homo sapiens