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Literature summary extracted from

  • Sorci, L.; Blaby, I.; De Ingeniis, J.; Gerdes, S.; Raffaelli, N.; de Crecy Lagard, V.; Osterman, A.
    Genomics-driven reconstruction of acinetobacter NAD metabolism: insights for antibacterial target selection (2010), J. Biol. Chem., 285, 39490-39499.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.1 expressed in Escherichia coli as a His-tagged fusion protein Acinetobacter baumannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.1 0.017
-
 deamido-NAD+ pH 7.5, 30°C Acinetobacter baumannii
6.3.5.1 4.3
-
 deamido-NMN pH 7.5, 30°C Acinetobacter baumannii

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.1 Acinetobacter baumannii
-
-
-
6.3.5.1 Acinetobacter baumannii B0V8W9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.1 using Ni-NTA chromatography Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.1 ATP + deamido-NAD+ + L-Gln
-
 Acinetobacter baumannii AMP + diphosphate + NAD+ + L-Glu
-
 ?
6.3.5.1 ATP + deamido-NAD+ + NH3 + H2O
-
 Acinetobacter baumannii AMP + diphosphate + NAD+
-
 ?
6.3.5.1 ATP + deamido-NMN + L-Gln poor substrate Acinetobacter baumannii AMP + diphosphate + NMN + L-Glu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.1 abNadE enzyme possesses an additional glutamine transferase domain Acinetobacter baumannii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.5.1 30
-
 assay at Acinetobacter baumannii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.1 0.0032
-
 deamido-NMN pH 7.5, 30°C Acinetobacter baumannii
6.3.5.1 0.57
-
 deamido-NAD+ pH 7.5, 30°C Acinetobacter baumannii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.1 7.5
-
 assay at Acinetobacter baumannii

General Information

EC Number General Information Comment Organism
6.3.5.1 malfunction nadE is dispensable when the nondeamidating salvage pathway functions as the only route of NAD biogenesis Acinetobacter baumannii
6.3.5.1 metabolism due to the presence of an additional glutamine transferase domain, abNadE can efficiently utilize l-glutamine (as well as ammonia) for the amidation of NAD precursor Acinetobacter baumannii
6.3.5.1 physiological function nadE, encoding glutamine-dependent NAD synthetase, is dispensable when the nondeamidating salvage pathway of nicotinamide salvage/recycling functions as the only route of NAD biogenesis Acinetobacter baumannii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.5.1 0.00074
-
 deamido-NMN pH 7.5, 30°C Acinetobacter baumannii
6.3.5.1 34
-
 deamido-NAD+ pH 7.5, 30°C Acinetobacter baumannii