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Literature summary extracted from

  • Fujimoto, Z.; Ichinose, H.; Maehara, T.; Honda, M.; Kitaoka, M.; Kaneko, S.
    Crystal structure of an exo-1,5-alpha-L-arabinofuranosidase from Streptomyces avermitilis provides insights into the mechanism of substrate discrimination between exo- and endo-type enzymes in glycoside hydrolase family 43 (2010), J. Biol. Chem., 285, 34134-34143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Escherichia coli BL21gold (DE3) cells Streptomyces avermitilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.55 sitting drop vapor diffusion method, using 0.8 M sodium citrate, 0.2 M sodium chloride, and 0.1 M Tris, pH 7.0 Streptomyces avermitilis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.55 D135A inactive Streptomyces avermitilis
3.2.1.55 D135N inactive Streptomyces avermitilis
3.2.1.55 D20A inactive Streptomyces avermitilis
3.2.1.55 D20N the mutant shows 0.7% of wild type activity Streptomyces avermitilis
3.2.1.55 E196A inactive Streptomyces avermitilis
3.2.1.55 L289A the mutant shows 10% of wild type activity Streptomyces avermitilis
3.2.1.55 N159A the mutant shows 1.5fold increased activity compared to the wild type Streptomyces avermitilis
3.2.1.55 N159L the mutant shows 19fold increased activity compared to the wild type Streptomyces avermitilis
3.2.1.55 Y192A the mutant shows 40% of wild type activity Streptomyces avermitilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.001
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.5
-
 4-nitrophenyl alpha-L-arabinofuranoside wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 1.1
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 1.7
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 11.5
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 40.7
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Streptomyces avermitilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces avermitilis 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 alpha-1,5-L-arabinofuranobiose + H2O
-
 Streptomyces avermitilis L-arabinofuranose
-
 ?
3.2.1.55 additional information the enzyme shows no activity towards alpha-1,2-L-arabinofuranobiose and alpha-1,3-L-arabinofuranobiose Streptomyces avermitilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
 Streptomyces avermitilis
3.2.1.55 Araf43A
-
 Streptomyces avermitilis
3.2.1.55 exo-1,5-alpha-L-arabinofuranosidase
-
 Streptomyces avermitilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 0.002
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.11
-
 4-nitrophenyl alpha-L-arabinofuranoside wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.56
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.62
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 1.07
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 4.55
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.55 0.00015
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme D20N, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.015
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.067
-
 alpha-1,5-L-arabinofuranobiose mutant enzyme L289A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.09
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.18
-
 alpha-1,5-L-arabinofuranobiose mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.22
-
 4-nitrophenyl alpha-L-arabinofuranoside wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 0.33
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 1.78
-
 alpha-1,5-L-arabinofuranobiose mutant enzyme N159A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 4.14
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant enzyme N159L, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 11.15
-
 alpha-1,5-L-arabinofuranobiose mutant enzyme Y192A, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis
3.2.1.55 63.6
-
 alpha-1,5-L-arabinofuranobiose wild type enzyme, in 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.0, at 30°C Streptomyces avermitilis