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Literature summary extracted from
- Cloning and characterization of oxidosqualene cyclases from Kalanchoe daigremontiana: enzymes catalyzing up to 10 rearrangement steps yielding friedelin and other triterpenoids (2010), J. Biol. Chem., 285, 29703-29712.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.8 | expressed in Saccharomyces cerevisiae mutant GIL77 cells | Kalanchoe daigremontiana |
| 5.4.99.49 | expressed in Saccharomyces cerevisiae | Kalanchoe daigremontiana |
| 5.4.99.50 | expressed in Saccharomyces cerevisiae strain GIL77 | Kalanchoe daigremontiana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.8 | Kalanchoe daigremontiana | - |
- |
- |
| 5.4.99.49 | Kalanchoe daigremontiana | E2IUA7 | - |
- |
| 5.4.99.50 | Kalanchoe daigremontiana | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.8 | epidermis | - |
Kalanchoe daigremontiana | - |
| 5.4.99.8 | leaf | - |
Kalanchoe daigremontiana | - |
| 5.4.99.49 | epidermis | the enzyme is expressed only in the epidermis layers lining the upper and lower surfaces of the leaf blade | Kalanchoe daigremontiana | - |
| 5.4.99.49 | leaf | - |
Kalanchoe daigremontiana | - |
| 5.4.99.50 | leaf epidermis | friedelin synthase is expressed only in the epidermis layers lining the upper and lower surfaces of the leaf blade | Kalanchoe daigremontiana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (S)-2,3-oxidosqualene | - |
Kalanchoe daigremontiana | cycloartenol | - |
? |  |
| 5.4.99.49 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Kalanchoe daigremontiana | glutinol | main product is glutinol with 66% yield, minor products are friedelin (14%), beta-amyrin (11%), and taraxerol (8%) | ? | |
| 5.4.99.50 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Kalanchoe daigremontiana | friedelin | 71% yield | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | CAS | - |
Kalanchoe daigremontiana |
| 5.4.99.8 | OSC | - |
Kalanchoe daigremontiana |
| 5.4.99.8 | Oxidosqualene cyclase | - |
Kalanchoe daigremontiana |
| 5.4.99.49 | GLS | - |
Kalanchoe daigremontiana |
| 5.4.99.50 | OSC | - |
Kalanchoe daigremontiana |
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Release 2023.1 (January 2023)
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