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Literature summary extracted from

  • Fernandez-Leiro, R.; Pereira-Rodriguez, A.; Cerdan, M.E.; Becerra, M.; Sanz-Aparicio, J.
    Structural analysis of Saccharomyces cerevisiae alpha-galactosidase and its complexes with natural substrates reveals new insights into substrate specificity of GH27 glycosidases (2010), J. Biol. Chem., 285, 28020-28033.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.22 crystal structures of tetrameric Saccharomyces cerevisiae alpha-galactosidase and its complexes with the substrates melibiose and raffinose have been determined to 1.95, 2.40, and 2.70 A resolution. The monomer folds into a catalytic (alpha/beta)8 barrel and a C-terminal beta-sandwich domain with unassigned function Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.22 A41Y kcat decreased compared to wild-tpye, Km (substrate: 4-nitrophenyl-alpha-D-galactopyranoside) or (substrate: melibiose) decreased compared to wild-type, Km (raffinose) increased compared to wild-type Saccharomyces cerevisiae
3.2.1.22 D149A mutant shows no activity Saccharomyces cerevisiae
3.2.1.22 D209A mutant shows no activity Saccharomyces cerevisiae
3.2.1.22 N252A mutant is highly unstable, no kinetic data available Saccharomyces cerevisiae
3.2.1.22 Q251A kcat and Km (substrate: 4-nitrophenyl-alpha-D-galactopyranoside) decreased compared to wild-type Saccharomyces cerevisiae
3.2.1.22 Q251A kcat decreased compared to wild-tpye, Km (substrate: 4-nitrophenyl-alpha-D-galactopyranoside) decreased compared to wild-type, Km (melibiose) increased compared to wild-type Saccharomyces cerevisiae
3.2.1.22 Y232R mutant is highly unstable, no kinetic data available Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.24
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant Q251W, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 1.4
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant Q251A, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 3.7
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 4.5
-
 4-nitrophenyl-alpha-D-galactopyranoside wild-type, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 5.8
-
 melibiose mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 11.2
-
 melibiose wild-type, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 54.1
-
 raffinose wild-type, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 87.9
-
 raffinose mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 254
-
 melibiose mutant Q251A, pH 7.4, 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 80000
-
 analytical ultracentrifugation under denaturing condition, monomer Saccharomyces cerevisiae
3.2.1.22 320000
-
 analytical ultracentrifugation under non-denaturing condition, tetramer Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Saccharomyces cerevisiae P04824
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 melibiose + H2O
-
 Saccharomyces cerevisiae alpha-D-galactose + alpha-D-glucose
-
 ?
3.2.1.22 raffinose + H2O
-
 Saccharomyces cerevisiae sucrose + alpha-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 tetramer analytical ultracentrifugation under non-denaturing condition, 4 * 80000 Da Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 alpha-galactosidase
-
 Saccharomyces cerevisiae
3.2.1.22 ScAGal
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 16
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant Q251W, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 46.8
-
 raffinose wild-type, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 90
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 91
-
 raffinose mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 113
-
 melibiose mutant Q251A, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 131
-
 melibiose mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 170
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant Q251A, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 193
-
 melibiose wild-type, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 286
-
 4-nitrophenyl-alpha-D-galactopyranoside wild-type, pH 7.4, 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 7.4
-
 assay at Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.22 0.45
-
 melibiose mutant Q251A, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 0.8
-
 raffinose mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 0.9
-
 raffinose wild-type, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 17
-
 melibiose wild-type, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 22
-
 melibiose mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 24
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant A41Y, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 63.5
-
 4-nitrophenyl-alpha-D-galactopyranoside wild-type, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 66
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant Q251W, pH 7.4, 30°C Saccharomyces cerevisiae
3.2.1.22 121
-
 4-nitrophenyl-alpha-D-galactopyranoside mutant Q251A, pH 7.4, 30°C Saccharomyces cerevisiae