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Literature summary extracted from

  • Okazaki, Y.; Ohshima, N.; Yoshizawa, I.; Kamei, Y.; Mariggio, S.; Okamoto, K.; Maeda, M.; Nogusa, Y.; Fujioka, Y.; Izumi, T.; Ogawa, Y.; Shiro, Y.; Wada, M.; Kato, N.; Corda, D.; Yanaka, N.
    A novel glycerophosphodiester phosphodiesterase, GDE5, controls skeletal muscle development via a non-enzymatic mechanism (2010), J. Biol. Chem., 285, 27652-27663.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.2 expression of C-terminally His6-tagged enzyme in Spodoptera frugiperda Sf9 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.2 additional information GDE5 RNAi gene silencing results in an increase in intracellular sn-glycero-3-phosphocholine levels. Construction of a truncated GDE5 construct, GDE5DELTAC471, which contains a GP-PDE sequence identified in other GP-PDEs but lacks GroPCho phosphodiesterase activity, and it shows a similar inhibitory effect on myogenic differentiation like the full-length enzyme, overview Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.2 Cd2+
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.2 Mg2+ activates, required Mus musculus
3.1.4.2 additional information no activation by Ca2+ Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.2 81000
-
 x * 81000, recombinant His6-tagged enzyme, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.2 sn-glycero-3-phosphocholine + H2O Mus musculus
-
 choline + sn-glycerol 3-phosphate
-
 ?
3.1.4.2 sn-glycero-3-phosphocholine + H2O Mus musculus C57BL/6
-
 choline + sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.2 Mus musculus
-
-
-
3.1.4.2 Mus musculus C57BL/6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.2 recombinant C-terminally His6-tagged enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography and gel filtration Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.2 skeletal muscle
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.2 2
-
 purified recombinant His-tagged GDE5, substrate is glycerophosphoethanolamine, pH 7.4, 37°C Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.2 glycerophosphoethanolamine + H2O
-
 Mus musculus ?
-
 ?
3.1.4.2 glycerophosphoethanolamine + H2O
-
 Mus musculus C57BL/6 ?
-
 ?
3.1.4.2 additional information the enzyme shows no significant activity with glycerophosphoglycerol, GroPIns, and glycerophosphoserine as substrates Mus musculus ?
-
 ?
3.1.4.2 additional information the enzyme shows no significant activity with glycerophosphoglycerol, GroPIns, and glycerophosphoserine as substrates Mus musculus C57BL/6 ?
-
 ?
3.1.4.2 sn-glycero-3-phosphocholine + H2O
-
 Mus musculus choline + sn-glycerol 3-phosphate
-
 ?
3.1.4.2 sn-glycero-3-phosphocholine + H2O
-
 Mus musculus C57BL/6 choline + sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.2 ? x * 81000, recombinant His6-tagged enzyme, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.2 GDE5
-
 Mus musculus
3.1.4.2 glycerophosphodiester phosphodiesterase
-
 Mus musculus
3.1.4.2 GP-PDE
-
 Mus musculus
3.1.4.2 More GDE5 is an UgpQ-type GDE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.2 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.2 7.4
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
3.1.4.2 malfunction GDE5 expression is reduced in atrophied skeletal muscles in mice and decreasing GDE5 abundance promotes myoblastic differentiation, suggesting that decreased GDE5 expression has a counter-regulatory effect on the progression of skeletal muscleatrophy. Transgenic mice specifically overexpressing GDE5DELTAC471 in skeletal muscle have less type II fiber-rich skeletal muscle mass. Forced expression of full-length GDE5 in cultured myoblasts suppresses myogenic differentiation. Transgenic mice specifically expressing GDE5DELTAC471 in skeletal muscle show less skeletal muscle mass, especially type II fiber-rich muscle Mus musculus
3.1.4.2 physiological function GDE5 selectively hydrolyzes glycerophosphocholine and controls skeletal muscle development. GDE5 negatively regulates skeletal muscle development even without GroPCho phosphodiesterase activity Mus musculus