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Literature summary extracted from

  • Fu, Z.; Runquist, J.A.; Montgomery, C.; Miziorko, H.M.; Kim, J.J.
    Functional insights into human HMG-CoA lyase from structures of Acyl-CoA-containing ternary complexes (2010), J. Biol. Chem., 285, 26341-26349.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.4 His-tagged version Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.4 vapor diffusion method Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.4 K48N inherited mutation Homo sapiens
4.1.3.4 K48Q mimics Lys acetylation Homo sapiens
4.1.3.4 R41M catalytically deficient Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.4 3-hydroxyglutaryl-CoA competitive inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.4 0.0134
-
3-hydroxy-3-methylglutaryl-CoA K48Q mutant protein, pH not specified in the publication, 30°C Homo sapiens
4.1.3.4 0.0209
-
3-hydroxy-3-methylglutaryl-CoA K48N mutant protein, pH not specified in the publication, 30°C Homo sapiens
4.1.3.4 0.0265
-
3-hydroxy-3-methylglutaryl-CoA wild type protein, pH not specified in the publication, 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.4 Mg2+
-
Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.4
-
Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.4 87.8
-
K48N mutant protein, pH not specified in the publication, 30°C Homo sapiens
4.1.3.4 100.3
-
K48Q mutant protein, pH not specified in the publication, 30°C Homo sapiens
4.1.3.4 136
-
wild type protein, pH not specified in the publication, 30°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 3-hydroxy-3-methylglutaryl-CoA
-
Homo sapiens acetoacetate + acetyl-CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.4 3-Hydroxy-3-methylglutaryl-CoA lyase
-
Homo sapiens