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Literature summary extracted from

  • Deng, X.; Lee, J.; Michael, A.J.; Tomchick, D.R.; Goldsmith, E.J.; Phillips, M.A.
    Evolution of substrate specificity within a diverse family of beta/alpha-barrel-fold basic amino acid decarboxylases: X-ray structure determination of enzymes with specificity for L-arginine and carboxynorspermidine (2010), J. Biol. Chem., 285, 25708-25719.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.19 expressed in Escherichia coli BL21(DE3) cells Vibrio vulnificus
4.1.1.96 expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.19 in complex with L-Arg, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.0, 0.2 M MgCl2, 12% (w/v) PEG-4000 Vibrio vulnificus
4.1.1.96 co-crystallized with 10 mM norspermidine, hanging drop vapor diffusion method, using 2.75 M ammonium sulfate, 0.1 M Bicine, pH 8.5 Campylobacter jejuni

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.96 K184E inactive Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.96 2.1
-
 carboxynorspermidine pH and temperature not specified in the publication Campylobacter jejuni
4.1.1.96 4.1
-
 carboxyspermidine pH and temperature not specified in the publication Campylobacter jejuni

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.19 Vibrio vulnificus Q7MK24
-
-
4.1.1.96 Campylobacter jejuni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.19 Ni2+-affinity column chromatography and gel filtration Vibrio vulnificus
4.1.1.96 Ni2+ affinity column chromatography and gel filtration Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.19 L-arginine
-
 Vibrio vulnificus agmatine + CO2
-
 ?
4.1.1.96 carboxynorspermidine
-
 Campylobacter jejuni bis(3-aminopropyl)amine + CO2
-
 ?
4.1.1.96 carboxyspermidine
-
 Campylobacter jejuni spermidine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.19 tetramer x-ray crystallography Vibrio vulnificus
4.1.1.96 homodimer x-ray crystallography Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
4.1.1.19 ADC
-
 Vibrio vulnificus
4.1.1.19 Biosynthetic arginine decarboxylase
-
 Vibrio vulnificus
4.1.1.96 CANSDC
-
 Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.96 0.24
-
 carboxyspermidine pH and temperature not specified in the publication Campylobacter jejuni
4.1.1.96 0.58
-
 carboxynorspermidine pH and temperature not specified in the publication Campylobacter jejuni

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.19 pyridoxal 5'-phosphate
-
 Vibrio vulnificus
4.1.1.96 pyridoxal 5'-phosphate
-
 Campylobacter jejuni