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Literature summary extracted from

  • Arent, S.; Christensen, C.E.; Pye, V.E.; N?rgaard, A.; Henriksen, A.
    The multifunctional protein in peroxisomal beta-oxidation: structure and substrate specificity of the Arabidopsis thaliana protein MFP2 (2010), J. Biol. Chem., 285, 24066-24077.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.17 recombinant expression of His-tagged AtMFP2 in Escherichia coli strain BL21(DE3) for the wild-type enzyme, and in Escherichia coli strain B834(DE3) for the selenomethionine-labeled variant Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.17 purified recombinant His-tagged wild-type and SeMet-labeled AtMFP2, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.17 peroxisome
-
 Arabidopsis thaliana 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.17 (3S)-3-hydroxyacyl-CoA Arabidopsis thaliana
-
 (E)-2(or 3)-enoyl-CoA + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.17 Arabidopsis thaliana Q9ZPI5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.17 recombinant His-tagged wild-type and SeMet-labeled AtMFP2 from Escherichia coli strains BL21(DE3) and B834(DE3), respectively, by nickel affinity chromatography and gel filtration Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.17 additional information
-
 2-enoyl-CoA substrate specificity of MFE2 and AIM, overview Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.17 (3S)-3-hydroxyacyl-CoA
-
 Arabidopsis thaliana (E)-2(or 3)-enoyl-CoA + H2O
-
 ?
4.2.1.17 additional information substrates are enoyl-CoA chains of C4-C14, neither AtMFP2 nor AtAIM1 efficiently degrade enoyl chains longer than C14-CoA, substrate specificity in vitro with 2-trans-enoyl-CoA substrates, AIM perfers th C4 substrate, while MFE2 prefers the C8 substrate, overview Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.17 AIM1
-
 Arabidopsis thaliana
4.2.1.17 MFP2
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.17 27
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.17 8.5
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.2.1.17 metabolism two multifunctional peroxisomal isozymes, MFP2 and AIM1, both with 2-trans-enoyl-CoA hydratase and L-3-hydroxyacyl-CoA dehydrogenase activities, function in Arabidopsis thaliana peroxisomal beta-oxidation, where fatty acids are degraded by the sequential removal of two carbon units Arabidopsis thaliana