Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schoenhofen, I.C.; Lunin, V.V.; Julien, J.P.; Li, Y.; Ajamian, E.; Matte, A.; Cygler, M.; Brisson, J.R.; Aubry, A.; Logan, S.M.; Bhatia, S.; Wakarchuk, W.W.; Young, N.M.
    Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori (2006), J. Biol. Chem., 281, 8907-8916.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.92 medicine the enzyme is involved biosynthesis of 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid (pseudaminic acid). Helicobacter pylori flagellin is heavily glycosylated with pseudaminic acid. The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Because motility is a key virulence factor for this and other important pathogens, the pseudaminic acid biosynthetic pathway offers potential for novel therapeutic targets Helicobacter pylori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.92 crystal structure of the native protein, its complexes with pyridoxal 5'-phosphate alone and in combination with UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine. A crystal of apo-PseC is obtained in 2 days at 20°C from a hanging drop by mixing 0.0015 ml of protein in buffer with 0.0015 ml of reservoir solution containing 0.2 M ammonium acetate, 0.1 M trisodium citrate, pH 5.6, 30% polyethylene glycol 4000. The crystals belong to space group P2(1)2(1)2 with unit cell dimensions a = 87.7, b = 155.4, and c = 71.5 A and Z = 4 Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate Helicobacter pylori the enzyme is involved biosynthesis of pseudaminic acid. Helicobacter pylori flagellin is heavily glycosylated with pseudaminic acid. The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Motility is a key virulence factor for this and other important pathogens UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.92 Helicobacter pylori O25130
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.92
-
 Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate
-
 Helicobacter pylori UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate the enzyme is involved biosynthesis of pseudaminic acid. Helicobacter pylori flagellin is heavily glycosylated with pseudaminic acid. The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Motility is a key virulence factor for this and other important pathogens Helicobacter pylori UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.92 homodimer PseC is a homodimer in solution (as shown by dynamic light scattering) as well as in the crystal structure, where the two monomers are related by 2fold crystallographic symmetry. The inter-subunit interactions are extensive, involving several elements of secondary structure, predominantly from the N-terminal domains of both monomers. Each monomer contributes to the active site Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.6.1.92 HP0366
-
 Helicobacter pylori
2.6.1.92 PseC
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.92 37
-
 assay at Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.92 7.2
-
 assay at Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.92 pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Helicobacter pylori

General Information

EC Number General Information Comment Organism
2.6.1.92 physiological function the enzyme is involved biosynthesis of 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid (pseudaminic acid). Helicobacter pylori flagellin is heavily glycosylated with pseudaminic acid. The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Motility is a key virulence factor for this and other important pathogens Helicobacter pylori