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Literature summary extracted from

  • Koivisto, P.; Robins, P.; Lindahl, T.; Sedgwick, B.
    Demethylation of 3-methylthymine in DNA by bacterial and human DNA dioxygenases (2004), J. Biol. Chem., 279, 40470-40474.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.33 AlkB is expressed in Escherichia coli BL21(DE3) cells Escherichia coli
1.14.11.33 isoforms ABH2 and ABH3 are expressed in baculovirus-infected Sf9 insect cells Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.33 Fe2+ dependent on Escherichia coli
1.14.11.33 Fe2+ dependent on Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.33 Escherichia coli
-
-
-
1.14.11.33 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.33 Ni-NTA agarose column chromatography Escherichia coli
1.14.11.33 Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylthymine + 2-oxoglutarate + O2 3-methylthymine residues in DNA is repaired inefficiently in vivo. The ABH3 protein repairs 3-methylthymine in both single-stranded and double-stranded polydeoxynucleotides, whereas the ABH2 protein prefers a duplex substrate Homo sapiens DNA-thymine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylthymine + 2-oxoglutarate + O2 3-methylthymine residues in DNA is repaired inefficiently in vivo. The AlkB protein repairs 3-methylthymine in both single-stranded and double-stranded polydeoxynucleotidese Escherichia coli DNA-thymine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated poly(deoxythymine) + 2-oxoglutarate + O2
-
 Escherichia coli poly(deoxythymine) + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 methylated poly(deoxythymine) + 2-oxoglutarate + O2
-
 Homo sapiens poly(deoxythymine) + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 additional information the enzyme does not demethylate 3-methyladenine, 7-methyladenine, 7-methylguanine, thymine (5-methyluracil), and 5-methylcytosine Escherichia coli ?
-
 ?
1.14.11.33 additional information the enzyme does not demethylate 3-methyladenine, 7-methyladenine, 7-methylguanine, thymine (5-methyluracil), and 5-methylcytosine Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.33 ABH2 isoform Homo sapiens
1.14.11.33 ABH3 isoform Homo sapiens
1.14.11.33 AlkB
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.33 6
-
 repair of DNA-3-methylthymine by AlkB is optimal at pH 6.0, but inefficient Escherichia coli
1.14.11.33 6.5
-
 repair of DNA-3-methylthymine by ABH3 is optimal at pH 6.5, but inefficient Homo sapiens
1.14.11.33 7.6 8 DNA-3-methylcytosine is repaired optimally at pH 7.5-8.0 Escherichia coli
1.14.11.33 7.6 8 DNA-3-methylcytosine is repaired optimally at pH 7.5-8.0 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.33 2-oxoglutarate
-
 Escherichia coli
1.14.11.33 2-oxoglutarate
-
 Homo sapiens