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Literature summary extracted from
- Minimal methylated substrate and extended substrate range of Escherichia coli AlkB protein, a 1-methyladenine-DNA dioxygenase (2003), J. Biol. Chem., 278, 44348-44354.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.33 | isoforms ABH2 and ABH3 are expressed in Sf9 insect cells | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.33 | 0.0014 | - |
poly(dm1A) | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.002 | - |
1-methyl-dAMP | apparent value, at 37°C, pH not specified in the publication | Escherichia coli |  |
| 1.14.11.33 | 0.0028 | - |
d(Tpm1ApT) | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.0044 | - |
d(Tpm1A) | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.0055 | - |
1-methyl-dATP | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.01 | - |
N1-methyl-ATP | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.012 | - |
2'-deoxy-1-methyl-adenosine 3'-phosphate | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.04 | 0.05 | d(Tpm1A) | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.33 | Fe2+ | required for activity | Escherichia coli | |
| 1.14.11.33 | Fe2+ | required for activity | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.33 | Escherichia coli | - |
- |
- |
| 1.14.11.33 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.33 | Ni-NTA column chromatography | Escherichia coli |
| 1.14.11.33 | Ni-NTA column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.33 | 1-methyl-dAMP + 2-oxoglutarate + O2 | - |
Escherichia coli | dAMP + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | 1-methyl-dAMP + 2-oxoglutarate + O2 | low activity | Homo sapiens | dAMP + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | 1-methyl-dATP + 2-oxoglutarate + O2 | - |
Escherichia coli | dATP + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | 1-methyl-dATP + 2-oxoglutarate + O2 | low activity | Homo sapiens | dATP + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | 2'-deoxy-1-methyl-adenosine 3'-phosphate + 2-oxoglutarate + O2 | - |
Escherichia coli | 2'-deoxyadenosine 3'-phosphate + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | 2'-deoxy-1-methyl-adenosine 3'-phosphate + 2-oxoglutarate + O2 | low activity | Homo sapiens | 2'-deoxyadenosine 3'-phosphate + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | d(Tpm1A) + 2-oxoglutarate + O2 | - |
Escherichia coli | d(TpA) + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | d(Tpm1A) + 2-oxoglutarate + O2 | low activity | Homo sapiens | d(TpA) + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | d(Tpm1ApT) + 2-oxoglutarate + O2 | - |
Escherichia coli | d(TpApT) + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | d(Tpm1ApT) + 2-oxoglutarate + O2 | low activity | Homo sapiens | d(TpApT) + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | N1-methyl-ATP + 2-oxoglutarate + O2 | - |
Escherichia coli | ATP + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | N1-methyl-ATP + 2-oxoglutarate + O2 | low activity | Homo sapiens | ATP + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | poly(dm1A) + 2-oxoglutarate + O2 | - |
Escherichia coli | poly(dA) + formaldehyde + succinate + CO2 | - |
? | |
| 1.14.11.33 | poly(dm1A) + 2-oxoglutarate + O2 | low activity | Homo sapiens | poly(dA) + formaldehyde + succinate + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.33 | 1-methyladenine-DNA dioxygenase | - |
Escherichia coli |
| 1.14.11.33 | 1-methyladenine-DNA dioxygenase | - |
Homo sapiens |
| 1.14.11.33 | ABH2 | isoform | Homo sapiens |
| 1.14.11.33 | ABH3 | isoform | Homo sapiens |
| 1.14.11.33 | AlkB | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.33 | 0.012 | - |
2'-deoxy-1-methyl-adenosine 3'-phosphate | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.02 | - |
N1-methyl-ATP | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.033 | - |
1-methyl-dATP | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.06 | - |
d(Tpm1A) | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.07 | - |
1-methyl-dAMP | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.12 | - |
d(Tpm1ApT) | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 0.2 | - |
poly(dm1A) | at 37°C, pH not specified in the publication | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.33 | 2-oxoglutarate | - |
Escherichia coli | |
| 1.14.11.33 | 2-oxoglutarate | - |
Homo sapiens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.33 | 1.7 | - |
1-methyl-ATP | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 3.3 | - |
2'-deoxy-1-methyl-adenosine 3'-phosphate | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 11.7 | - |
1-methyl-dATP | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 35 | - |
1-methyl-dAMP | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 43 | - |
d(Tpm1ApT) | at 37°C, pH not specified in the publication | Escherichia coli | |
| 1.14.11.33 | 143 | - |
poly(dm1A) | at 37°C, pH not specified in the publication | Escherichia coli |
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