Any feedback?
Literature summary extracted from
- Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose in Aneurinibacillus thermoaerophilus L420-91T (2003), J. Biol. Chem., 278, 26410-26417.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.1.197 | overexpression in Escherichia coli | Aneurinibacillus thermoaerophilus |
| 2.6.1.90 | overexpression in Escherichia coli | Aneurinibacillus thermoaerophilus |
| 5.3.2.3 | overexpression in Escherichia coli | Aneurinibacillus thermoaerophilus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.3.1.197 | to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol | Aneurinibacillus thermoaerophilus |
| 2.6.1.90 | to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol | Aneurinibacillus thermoaerophilus |
| 5.3.2.3 | to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol | Aneurinibacillus thermoaerophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.197 | 0.061 | - |
acetyl-CoA | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus |  |
| 2.3.1.197 | 0.0667 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.6.1.90 | additional information | metal ions are not required | Aneurinibacillus thermoaerophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.6.1.90 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | Aneurinibacillus thermoaerophilus | the glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtB is involved in this pathway | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? | |
| 2.6.1.90 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | the glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtB is involved in this pathway | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? | |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | Aneurinibacillus thermoaerophilus | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.1.197 | Aneurinibacillus thermoaerophilus | Q6T1W7 | - |
- |
| 2.6.1.90 | Aneurinibacillus thermoaerophilus | Q6T1W6 | - |
- |
| 2.6.1.90 | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | Q6T1W6 | - |
- |
| 5.3.2.3 | Aneurinibacillus thermoaerophilus | Q6T1W8 | - |
- |
| 5.3.2.3 | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | Q6T1W8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.1.197 | - |
Aneurinibacillus thermoaerophilus |
| 2.6.1.90 | - |
Aneurinibacillus thermoaerophilus |
| 5.3.2.3 | - |
Aneurinibacillus thermoaerophilus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 2.6.1.90 | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate = dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | the reaction occurs in the reverse direction | Aneurinibacillus thermoaerophilus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.3.1.197 | 4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity | Aneurinibacillus thermoaerophilus |
| 2.6.1.90 | 4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity | Aneurinibacillus thermoaerophilus |
| 5.3.2.3 | 4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity | Aneurinibacillus thermoaerophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.197 | acetyl-CoA + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose | the enzyme is involved in the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose. The reverse reaction cannot be detected | Aneurinibacillus thermoaerophilus | CoA + dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose | - |
? | |
| 2.6.1.90 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | - |
Aneurinibacillus thermoaerophilus | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? | |
| 2.6.1.90 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | the glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtB is involved in this pathway | Aneurinibacillus thermoaerophilus | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? | |
| 2.6.1.90 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | - |
Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? | |
| 2.6.1.90 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | the glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtB is involved in this pathway | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? | |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway | Aneurinibacillus thermoaerophilus | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The activity of FdtA is monitored by a simultaneous incubation with dTDP-6-deoxy-D-xylohex-4-ulose with FdtB and the required cosubstrates. The resulting product, dTDP-D-Fucp3N, is detectable by HPLC analysis | Aneurinibacillus thermoaerophilus | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The activity of FdtA is monitored by a simultaneous incubation with dTDP-6-deoxy-D-xylohex-4-ulose with FdtB and the required cosubstrates. The resulting product, dTDP-D-Fucp3N, is detectable by HPLC analysis | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.1.197 | dTDP-D-Fucp3N acetylase | - |
Aneurinibacillus thermoaerophilus |
| 2.3.1.197 | FdtC | - |
Aneurinibacillus thermoaerophilus |
| 2.6.1.90 | FdtB | - |
Aneurinibacillus thermoaerophilus |
| 5.3.2.3 | FdtA | - |
Aneurinibacillus thermoaerophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.197 | 37 | - |
assay at | Aneurinibacillus thermoaerophilus |
| 2.3.1.197 | 50 | 55 | - |
Aneurinibacillus thermoaerophilus |
| 2.6.1.90 | 37 | - |
assay at | Aneurinibacillus thermoaerophilus |
| 2.6.1.90 | 50 | 55 | - |
Aneurinibacillus thermoaerophilus |
| 5.3.2.3 | 37 | - |
assay at | Aneurinibacillus thermoaerophilus |
| 5.3.2.3 | 50 | 55 | - |
Aneurinibacillus thermoaerophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.197 | 2.3 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus | |
| 2.3.1.197 | 3.1 | - |
acetyl-CoA | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.197 | 7.4 | - |
assay at | Aneurinibacillus thermoaerophilus |
| 2.6.1.90 | 7.4 | - |
assay at | Aneurinibacillus thermoaerophilus |
| 5.3.2.3 | 7.4 | - |
assay at | Aneurinibacillus thermoaerophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.6.1.90 | pyridoxal 5'-phosphate | - |
Aneurinibacillus thermoaerophilus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.197 | 3.4 | - |
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus | |
| 2.3.1.197 | 50.8 | - |
acetyl-CoA | pH 7.4, 37°C | Aneurinibacillus thermoaerophilus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
