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Literature summary extracted from

  • Zhang, L.; Kudo, T.; Takaya, N.; Shoun, H.
    The B helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH (2002), J. Biol. Chem., 277, 33842-33847.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.14 expressed in Escherichia coli JM109 (DE3) cells Cutaneotrichosporon cutaneum
1.7.1.14 expressed in Escherichia coli JM109 (DE3) cells Fusarium oxysporum

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.14 G76A the mutation decreases the NADH-dependent activity to 40% of the wild type activity Fusarium oxysporum
1.7.1.14 K77A the mutation decreases the NADH-dependent activity Fusarium oxysporum
1.7.1.14 Q78A the mutation slightly improves the NADH-dependent activity Fusarium oxysporum
1.7.1.14 S73A the mutation decreases the NADH-dependent activity Fusarium oxysporum
1.7.1.14 S73G/S75G the double mutation significantly improves NADPH-dependent Nor activity without affecting, or even slightly increasing, the NADHd-ependent activity Fusarium oxysporum
1.7.1.14 S75A the mutation decreases the NADH-dependent activity Fusarium oxysporum
1.7.1.14 S75G the mutation significantly improves the NADPH-dependent Nor activity of isozyme Fnor, and the overall activity is accelerated via the NADPH-enhanced reduction step Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.14 Cutaneotrichosporon cutaneum
-
-
-
1.7.1.14 Fusarium oxysporum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.14 DEAE-cellulose column chromatography and Mono Q or Resource Q column chromatography Cutaneotrichosporon cutaneum
1.7.1.14 DEAE-cellulose column chromatography and Mono Q or Resource Q column chromatography Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.14 NO + NADH + H+ isozyme Fnor utilizes only NADH as electron donor Fusarium oxysporum N2O + NAD+ + H2O
-
 ?
1.7.1.14 NO + NADH + H+ isozyme Tnor utilizes both NADH and NADPH as electron donors Cutaneotrichosporon cutaneum N2O + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.14 cytochrome P450nor
-
 Cutaneotrichosporon cutaneum
1.7.1.14 cytochrome P450nor
-
 Fusarium oxysporum
1.7.1.14 Fnor isozyme Fusarium oxysporum
1.7.1.14 NOR
-
 Cutaneotrichosporon cutaneum
1.7.1.14 NOR
-
 Fusarium oxysporum
1.7.1.14 P450nor
-
 Cutaneotrichosporon cutaneum
1.7.1.14 P450nor
-
 Fusarium oxysporum
1.7.1.14 Tnor isozyme Cutaneotrichosporon cutaneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.14 heme
-
 Cutaneotrichosporon cutaneum
1.7.1.14 heme
-
 Fusarium oxysporum
1.7.1.14 NADH isozyme Fnor utilizes only NADH as electron donor Fusarium oxysporum
1.7.1.14 NADH isozyme Tnor utilizes both NADH and NADPH as electron donors Cutaneotrichosporon cutaneum
1.7.1.14 NADPH isozyme Tnor utilizes both NADH and NADPH as electron donors Cutaneotrichosporon cutaneum