BRENDA - Enzyme Database show

Cytochrome P450 CYP79B2 from Arabidopsis catalyzes the conversion of tryptophan to indole-3-acetaldoxime, a precursor of indole glucosinolates and indole-3-acetic acid

Mikkelsen, M.; Hansen, C.; Wittstock, U.; Halkier, B.; J. Biol. Chem. 275, 33712-33717 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.156
expressed in Escherichia coli C43(DE3) cells
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.156
1-aminobenzotriazole
the activity of CYP79B2 is inhibited 18 and 75% by 0.4 mM and 4 mM 1-aminobenzotriazole
Arabidopsis thaliana
1.14.14.156
2,4-Dichlorophenol
800 mM 2,4-dichlorophenol added to the CYP79B2 reconstitution assay inhibits the activity by 72%
Arabidopsis thaliana
1.14.14.156
H2O2
100 mM H2O2 added to the CYP79B2 reconstitution assay inhibits the activity by 96%
Arabidopsis thaliana
1.14.14.156
MnCl2
1 mM MnCl2 added to the CYP79B2 reconstitution assay inhibits the activity by 34%
Arabidopsis thaliana
1.14.14.156
additional information
CYP79B2 is not inhibited by Me2SO of a maximum concentration of 4% (v/v); the combination of 100 mM H2O2, 1 mM MnCl2, and 800 mM 2,4-dichlorophenol added to the reconstitution assay inhibits the CYP79B2 activity by 99%
Arabidopsis thaliana
1.14.14.156
tetcyclasis
the activity of CYP79B2 is inhibited 54 and 76% by 0.02 and 0.2 mM tetcyclasis
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.156
0.021
-
L-tryptophan
in 100 mM Tricine, pH 7.9, at 34°C
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.156
60000
-
SDS-PAGE
Arabidopsis thaliana
1.14.14.156
61000
-
estimated from amino acid sequence
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.156
Arabidopsis thaliana
O81346
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.156
flower
-
Arabidopsis thaliana
-
1.14.14.156
leaf
-
Arabidopsis thaliana
-
1.14.14.156
root
highest expression in roots
Arabidopsis thaliana
-
1.14.14.156
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.156
L-tryptophan + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
715448
Arabidopsis thaliana
(E)-indol-3-ylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.156
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.156
expressed in Escherichia coli C43(DE3) cells
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.156
NADPH
-
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.156
1-aminobenzotriazole
the activity of CYP79B2 is inhibited 18 and 75% by 0.4 mM and 4 mM 1-aminobenzotriazole
Arabidopsis thaliana
1.14.14.156
2,4-Dichlorophenol
800 mM 2,4-dichlorophenol added to the CYP79B2 reconstitution assay inhibits the activity by 72%
Arabidopsis thaliana
1.14.14.156
H2O2
100 mM H2O2 added to the CYP79B2 reconstitution assay inhibits the activity by 96%
Arabidopsis thaliana
1.14.14.156
MnCl2
1 mM MnCl2 added to the CYP79B2 reconstitution assay inhibits the activity by 34%
Arabidopsis thaliana
1.14.14.156
additional information
CYP79B2 is not inhibited by Me2SO of a maximum concentration of 4% (v/v); the combination of 100 mM H2O2, 1 mM MnCl2, and 800 mM 2,4-dichlorophenol added to the reconstitution assay inhibits the CYP79B2 activity by 99%
Arabidopsis thaliana
1.14.14.156
tetcyclasis
the activity of CYP79B2 is inhibited 54 and 76% by 0.02 and 0.2 mM tetcyclasis
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.156
0.021
-
L-tryptophan
in 100 mM Tricine, pH 7.9, at 34°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.156
60000
-
SDS-PAGE
Arabidopsis thaliana
1.14.14.156
61000
-
estimated from amino acid sequence
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.156
flower
-
Arabidopsis thaliana
-
1.14.14.156
leaf
-
Arabidopsis thaliana
-
1.14.14.156
root
highest expression in roots
Arabidopsis thaliana
-
1.14.14.156
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.156
L-tryptophan + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
715448
Arabidopsis thaliana
(E)-indol-3-ylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.14.14.156
Arabidopsis thaliana
CYP79B2 is wound-inducible
up
General Information
EC Number
General Information
Commentary
Organism
1.14.14.156
metabolism
CYP79B2 is involved in indole glucosinolate biosynthesis. CYP79B2 functions in biosynthesis of indole-3-acetic acid
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.156
metabolism
CYP79B2 is involved in indole glucosinolate biosynthesis. CYP79B2 functions in biosynthesis of indole-3-acetic acid
Arabidopsis thaliana
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.14.156
Arabidopsis thaliana
CYP79B2 is wound-inducible
up