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Literature summary extracted from

  • Mikkelsen, M.; Hansen, C.; Wittstock, U.; Halkier, B.
    Cytochrome P450 CYP79B2 from Arabidopsis catalyzes the conversion of tryptophan to indole-3-acetaldoxime, a precursor of indole glucosinolates and indole-3-acetic acid (2000), J. Biol. Chem., 275, 33712-33717.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.156 expressed in Escherichia coli C43(DE3) cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.156 1-aminobenzotriazole the activity of CYP79B2 is inhibited 18 and 75% by 0.4 mM and 4 mM 1-aminobenzotriazole Arabidopsis thaliana
1.14.14.156 2,4-Dichlorophenol 800 mM 2,4-dichlorophenol added to the CYP79B2 reconstitution assay inhibits the activity by 72% Arabidopsis thaliana
1.14.14.156 H2O2 100 mM H2O2 added to the CYP79B2 reconstitution assay inhibits the activity by 96% Arabidopsis thaliana
1.14.14.156 MnCl2 1 mM MnCl2 added to the CYP79B2 reconstitution assay inhibits the activity by 34% Arabidopsis thaliana
1.14.14.156 additional information CYP79B2 is not inhibited by Me2SO of a maximum concentration of 4% (v/v); the combination of 100 mM H2O2, 1 mM MnCl2, and 800 mM 2,4-dichlorophenol added to the reconstitution assay inhibits the CYP79B2 activity by 99% Arabidopsis thaliana
1.14.14.156 tetcyclasis the activity of CYP79B2 is inhibited 54 and 76% by 0.02 and 0.2 mM tetcyclasis Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.156 0.021
-
 L-tryptophan in 100 mM Tricine, pH 7.9, at 34°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.156 60000
-
 SDS-PAGE Arabidopsis thaliana
1.14.14.156 61000
-
 estimated from amino acid sequence Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.156 Arabidopsis thaliana O81346
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.156 flower
-
 Arabidopsis thaliana
-
1.14.14.156 leaf
-
 Arabidopsis thaliana
-
1.14.14.156 root highest expression in roots Arabidopsis thaliana
-
1.14.14.156 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.156 L-tryptophan + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Arabidopsis thaliana (E)-indol-3-ylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.156 CYP79B2
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.156 NADPH
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
1.14.14.156 Arabidopsis thaliana CYP79B2 is wound-inducible up

General Information

EC Number General Information Comment Organism
1.14.14.156 metabolism CYP79B2 is involved in indole glucosinolate biosynthesis. CYP79B2 functions in biosynthesis of indole-3-acetic acid Arabidopsis thaliana