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Literature summary extracted from

  • Park, J.K.; Keyhani, N.O.; Roseman, S.
    Chitin catabolism in the marine bacterium Vibrio furnissii. Identification, molecular cloning, and characterization of A N,N'-diacetylchitobiose phosphorylase (2000), J. Biol. Chem., 275, 33077-33083.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.280 expression in Escherichia coli Vibrio furnissii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.280 0.51
-
 phosphate pH 7.0, 30°C Vibrio furnissii
2.4.1.280 0.58
-
 N,N'-diacetylchitobiose pH 7.0, 30°C Vibrio furnissii
2.4.1.280 0.93
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.0, 30°C, reverse phosphorolysis reaction Vibrio furnissii
2.4.1.280 1
-
 N-acetyl-D-glucosamine pH 7.0, 30°C, reverse phosphorolysis reaction Vibrio furnissii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.280 cytosol
-
 Vibrio furnissii 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.280 additional information no requirement for divalent cations Vibrio furnissii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.280 89670
-
 calculated from amino acid sequence Vibrio furnissii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.280 N,N'-diacetylchitobiose + phosphate Vibrio furnissii
-
 N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.4.1.280 N,N'-diacetylchitobiose + phosphate Vibrio furnissii
-
 N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate inversion of the anomeric configuration r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.280 Vibrio furnissii Q9F8X1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.280
-
 Vibrio furnissii

Storage Stability

EC Number Storage Stability Organism
2.4.1.280 -20°C or -80°C Vibrio furnissii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.280 4-nitrophenyl N,N'-diacetylchitobioside + phosphate reaction at 8.5% the rate of N,N'-diacetylchitobiose Vibrio furnissii N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-D-glucosaminide
-
 r
2.4.1.280 additional information no substrates are N-acetylchitooligosaccharides, cellobiose, trehalose, lactose, maltose or sucrose Vibrio furnissii ?
-
 ?
2.4.1.280 N,N'-diacetylchitobiose + phosphate
-
 Vibrio furnissii N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.4.1.280 N,N'-diacetylchitobiose + phosphate
-
 Vibrio furnissii N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate inversion of the anomeric configuration r
2.4.1.280 N-acetyl-alpha-D-glucosamine 1-phosphate + 4-nitrophenyl N-acetyl-beta-D-glucosaminide reverse phosphorolysis, reaction at 36% the rate of N-acetyl-D-glucosamine Vibrio furnissii 4-nitrophenyl N,N'-diacetylchitobioside + phosphate
-
 r
2.4.1.280 N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate reverse phosphorolysis reaction Vibrio furnissii N,N'-diacetylchitobiose + phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.280 chbP
-
 Vibrio furnissii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.280 20 37
-
 Vibrio furnissii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.280 4 37 1 h stable Vibrio furnissii
2.4.1.280 50
-
 1 h 50% loss of activity Vibrio furnissii
2.4.1.280 70
-
 1 h compete inactivation Vibrio furnissii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.280 6.5 7
-
 Vibrio furnissii