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Literature summary extracted from

  • Cartwright, J.L.; McLennan, A.G.
    The Saccharomyces cerevisiae YOR163w gene encodes a diadenosine 5', 5"'-P1,P6-hexaphosphate (Ap6A) hydrolase member of the MutT motif (Nudix hydrolase) family (1999), J. Biol. Chem., 274, 8604-8610.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.60 dithiothreitol absolute requirement for a reducing agent, such as dithiothreitol (1 mM) Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.60 expression in Escherichia coli and Saccharomyces cerevisiae Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.60 Ca2+
-
Saccharomyces cerevisiae
3.6.1.60 F- noncompetitive inhibitor Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.60 Mg2+ optimal activity with 4-10 mM Mg2+ or 0.2 mM Mn2+ Saccharomyces cerevisiae
3.6.1.60 Mn2+ optimal activity with 4-10 mM Mg2+ or 0.2 mM Mn2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.60 21443
-
x * 21443, calculation from sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O Saccharomyces cerevisiae the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation adenosine 5'-tetraphosphate + AMP
-
?
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O Saccharomyces cerevisiae the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation adenosine 5'-tetraphosphate + ADP
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.60 Saccharomyces cerevisiae Q99321
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.60 additional information no activity with P1,P4-bis(5'-adenosyl)tetraphosphate or other dinucleotides, mononucleotides, nucleotide sugars, or nucleotide alcohols Saccharomyces cerevisiae ?
-
?
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation Saccharomyces cerevisiae adenosine 5'-tetraphosphate + AMP
-
?
3.6.1.60 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O hydrolysis of P1,P5-bis(5'-adenosyl)pentaphosphate yields predominantly AMP and adenosine tetraphosphate (96%) Saccharomyces cerevisiae adenosine 5'-tetraphosphate + AMP
-
?
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation Saccharomyces cerevisiae adenosine 5'-tetraphosphate + ADP
-
?
3.6.1.60 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O hydrolysis in H218O shows that ADP and adenosine 5*-tetraphosphate are produced by attack at Pbeta and AMP and adenosine 5'-pentaphosphate are produced by attack at Palpha (76%) and AMP and adenosine 5'-pentaphosphate are produced by attack at Palpha (24%). P1,P6-bis(5'-adenosyl)hexaphosphate is a 8fold better substrate than P1,P5-bis(5'-adenosyl)pentaphosphate Saccharomyces cerevisiae adenosine 5'-tetraphosphate + ADP
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.60 ? x * 21443, calculation from sequence Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.60 50
-
-
Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.60 6.9
-
-
Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.60 0.08
-
F- pH 6.9, 37°C Saccharomyces cerevisiae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.60 3.3
-
pH 6.9, 37°C Saccharomyces cerevisiae Ca2+

Expression

EC Number Organism Comment Expression
3.6.1.60 Saccharomyces cerevisiae expressed in log phase yeast cells up