EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.60 | dithiothreitol | absolute requirement for a reducing agent, such as dithiothreitol (1 mM) | Saccharomyces cerevisiae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.60 | expression in Escherichia coli and Saccharomyces cerevisiae | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.60 | Ca2+ | - |
Saccharomyces cerevisiae | |
3.6.1.60 | F- | noncompetitive inhibitor | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.60 | Mg2+ | optimal activity with 4-10 mM Mg2+ or 0.2 mM Mn2+ | Saccharomyces cerevisiae | |
3.6.1.60 | Mn2+ | optimal activity with 4-10 mM Mg2+ or 0.2 mM Mn2+ | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.60 | 21443 | - |
x * 21443, calculation from sequence | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.60 | P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | Saccharomyces cerevisiae | the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation | adenosine 5'-tetraphosphate + AMP | - |
? | |
3.6.1.60 | P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | Saccharomyces cerevisiae | the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation | adenosine 5'-tetraphosphate + ADP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.60 | Saccharomyces cerevisiae | Q99321 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.60 | additional information | no activity with P1,P4-bis(5'-adenosyl)tetraphosphate or other dinucleotides, mononucleotides, nucleotide sugars, or nucleotide alcohols | Saccharomyces cerevisiae | ? | - |
? | |
3.6.1.60 | P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation | Saccharomyces cerevisiae | adenosine 5'-tetraphosphate + AMP | - |
? | |
3.6.1.60 | P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | hydrolysis of P1,P5-bis(5'-adenosyl)pentaphosphate yields predominantly AMP and adenosine tetraphosphate (96%) | Saccharomyces cerevisiae | adenosine 5'-tetraphosphate + AMP | - |
? | |
3.6.1.60 | P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | the function of the enzyme may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation | Saccharomyces cerevisiae | adenosine 5'-tetraphosphate + ADP | - |
? | |
3.6.1.60 | P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | hydrolysis in H218O shows that ADP and adenosine 5*-tetraphosphate are produced by attack at Pbeta and AMP and adenosine 5'-pentaphosphate are produced by attack at Palpha (76%) and AMP and adenosine 5'-pentaphosphate are produced by attack at Palpha (24%). P1,P6-bis(5'-adenosyl)hexaphosphate is a 8fold better substrate than P1,P5-bis(5'-adenosyl)pentaphosphate | Saccharomyces cerevisiae | adenosine 5'-tetraphosphate + ADP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.60 | ? | x * 21443, calculation from sequence | Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.60 | 50 | - |
- |
Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.60 | 6.9 | - |
- |
Saccharomyces cerevisiae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.60 | 0.08 | - |
F- | pH 6.9, 37°C | Saccharomyces cerevisiae |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.6.1.60 | 3.3 | - |
pH 6.9, 37°C | Saccharomyces cerevisiae | Ca2+ |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.6.1.60 | Saccharomyces cerevisiae | expressed in log phase yeast cells | up |