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Literature summary extracted from
- Sulfide-quinone reductase from Rhodobacter capsulatus. Purification, cloning, and expression (1997), J. Biol. Chem., 272, 9890-9894.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.4 | expressed in Escherichia coli BL21(DE3) cells | Rhodobacter capsulatus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.4 | 0.002 | - |
Sulfide | in 10 mM bis-Tris-HCl, pH 6.5, temperature not specified in the publication | Rhodobacter capsulatus |  |
| 1.8.5.4 | 0.002 | - |
decylubiquinone | in 10 mM bis-Tris-HCl, pH 6.5, temperature not specified in the publication | Rhodobacter capsulatus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.5.4 | membrane | - |
Rhodobacter capsulatus | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.4 | 47000 | - |
calculated from amino acid sequence | Rhodobacter capsulatus |
| 1.8.5.4 | 55000 | - |
SDS-PAGE | Rhodobacter capsulatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.4 | Rhodobacter capsulatus | Q52722 | - |
- |
| 1.8.5.4 | Rhodobacter capsulatus DSM 155 | Q52722 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.5.4 | DEAE-cellulose column chromatography and Mono Q column chromatography | Rhodobacter capsulatus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.4 | 0.1 | - |
crude extract, in 10 mM bis-Tris-HCl, pH 6.5, temperature not specified in the publication | Rhodobacter capsulatus |
| 1.8.5.4 | 3.5 | - |
after 35fold purification, in 10 mM bis-Tris-HCl, pH 6.5, temperature not specified in the publication | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.4 | sulfide + decylubiquinone | - |
Rhodobacter capsulatus | sulfur + decylubiquinol | - |
? | |
| 1.8.5.4 | sulfide + decylubiquinone | - |
Rhodobacter capsulatus DSM 155 | sulfur + decylubiquinol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.4 | SQR | - |
Rhodobacter capsulatus |
| 1.8.5.4 | sulfide-quinone reductase | - |
Rhodobacter capsulatus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.4 | 6.3 | - |
- |
Rhodobacter capsulatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.5.4 | FAD | - |
Rhodobacter capsulatus | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.8.5.4 | Rhodobacter capsulatus | calculated from amino acid sequence | - |
9.7 |
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