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Literature summary extracted from

  • Nagy, J.M.; Cass, A.E.; Brown, K.A.
    Purification and characterization of recombinant catalase-peroxidase, which confers isoniazid sensitivity in Mycobacterium tuberculosis (1997), J. Biol. Chem., 272, 31265-31271.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli strain UM255 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.96
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, using tert-butyl hydroperoxide as cosubstrate, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C Mycobacterium tuberculosis
1.11.1.21 30
-
 H2O2 catalatic activity, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.21 80000
-
 2 * 80000, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 DEAE-Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + tert-butyl hydroperoxide
-
 Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + ?
-
 ?
1.11.1.21 H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.21 homodimer 2 * 80000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 4.5
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidatic activity, using tert-butyl hydroperoxide as cosubstrate, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C Mycobacterium tuberculosis
1.11.1.21 2300
-
 H2O2 catalatic activity, in 10 mM K2HPO4/KH2PO4 (pH 7.5), at 25°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme the enzyme contains approximately 0.5 heme per dimer Mycobacterium tuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.21 Mycobacterium tuberculosis isoelectric focusing 3.8 3.7

General Information

EC Number General Information Comment Organism
1.11.1.21 physiological function catalase-peroxidase confers isoniazid (isonicotinic acid hydrazide) sensitivity in Mycobacterium tuberculosis Mycobacterium tuberculosis