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Literature summary extracted from

  • Hammarloef, D.L.; Liljas, L.; Hughes, D.
    Temperature-sensitive mutants of RNase E in Salmonella enterica (2011), J. Bacteriol., 193, 6639-6650.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.12 A327P site-directed mutagensis, temperature-sensitive mutant Salmonella enterica
3.1.26.12 A327P mutation in N-terminal part of enzyme, temperature-sensitive mutation that is able to suppress the slow growth caused by the mutation tufA499 at permissive temperatures. In addition, mutation causes a large increase 503 in rne mRNA steady state levels Salmonella enterica
3.1.26.12 A448V site-directed mutagensis, the mutation causes steric problemes and leads to conformational changes Salmonella enterica
3.1.26.12 C471Y site-directed mutagensis, the mutation causes steric problemes and leads to conformational changes Salmonella enterica
3.1.26.12 G113D site-directed mutagensis, the mutation causes steric problemes and leads to conformational changes Salmonella enterica
3.1.26.12 G66C site-directed mutagensis, temperature-sensitive mutant, the mutation causes steric problemes and leads to conformational changes Salmonella enterica
3.1.26.12 G66C mutation in N-terminal part of enzyme, temperature-sensitive mutation that is able to suppress the slow growth caused by the mutation tufA499 at permissive temperatures. In addition, mutation causes a large increase 503 in rne mRNA steady state levels Salmonella enterica
3.1.26.12 I207N site-directed mutagensis, temperature-sensitive mutant, the mutation reduces the nonpolar contacts in the core, which may lead to a less stable protein Salmonella enterica
3.1.26.12 I207N mutation in N-terminal part of enzyme, temperature-sensitive mutation that is able to suppress the slow growth caused by the mutation tufA499 at permissive temperatures. In addition, mutation causes a large increase 503 in rne mRNA steady state levels Salmonella enterica
3.1.26.12 I207S site-directed mutagensis, temperature-sensitive mutant, the mutation reduces the nonpolar contacts in the core, which may lead to a less stable protein Salmonella enterica
3.1.26.12 I207S mutation in N-terminal part of enzyme, temperature-sensitive mutation that is able to suppress the slow growth caused by the mutation tufA499 at permissive temperatures. In addition, mutation causes a large increase 503 in rne mRNA steady state levels Salmonella enterica
3.1.26.12 L424R site-directed mutagensis, the mutation reduces the nonpolar contacts in the core, which may lead to a less stable protein Salmonella enterica
3.1.26.12 additional information identification of the EF-Tu mutation, tufA499, with a slow-groth phenotype, structural basis of properties of suppressors of the mutation, overview. Isolation and identification of temperature-sensitive mutations in RNase E that suppress the slow growth of tufA499. Mutations in rne affect the steady-state level of RNase E mRNA. The rne ts and ts suppressor mutations affect 9S to 5S rRNA processing, growrh profiles, overview. The ts mutations in RNase E affect the maturation of hisR tRNAHis Salmonella enterica
3.1.26.12 V459G site-directed mutagensis, the mutation reduces the nonpolar contacts in the core, which may lead to a less stable protein Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.12 Salmonella enterica
-
-
-
3.1.26.12 Salmonella enterica
-
 serovar typhimurium
-

Synonyms

EC Number Synonyms Comment Organism
3.1.26.12 RNase E
-
 Salmonella enterica

General Information

EC Number General Information Comment Organism
3.1.26.12 physiological function RNase E is a key component of the RNA degradosome, a multienzyme complex hat plays a central role in mRNA turnover and the processing of stable RNA in eubacteria. The degradosome is composed of a tetramer of RNase E molecules interacting via their N-terminal regions, with the C-terminal end of each RNase E molecule complexed with a monomer of RhlB, a dimer of enolase, and a trimer of PNPase Salmonella enterica