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Literature summary extracted from

  • Fan, C.; Bobik, T.A.
    The N-terminal region of the medium subunit (PduD) packages adenosylcobalamin-dependent diol dehydratase (PduCDE) into the Pdu microcompartment (2011), J. Bacteriol., 193, 5623-5628.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.28 expression of fusion protein PduD1-18-eGFP from a plasmid in a pduCDE deletion mutant Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.28 additional information construction of a fusion protein PduD1-18-eGFP Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.28 propane-1,2-diol Salmonella enterica
-
 propanal + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Salmonella enterica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.28 native enzyme by repeated immunoprecipitation on protein A-Sepharose beads, proteins fused to the N-terminus of PduD are encapsulated within the shell of the Pdu MCP Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 propane-1,2-diol
-
 Salmonella enterica propanal + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.28 coenzyme B12-dependent diol dehydratase
-
 Salmonella enterica
4.2.1.28 diol dehydratase
-
 Salmonella enterica
4.2.1.28 PduCDE
-
 Salmonella enterica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.28 37
-
 assay at Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.28 7.5
-
 assay at Salmonella enterica

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 adenosylcobalamin i.e. coenzyme B12, dependent on Salmonella enterica

General Information

EC Number General Information Comment Organism
4.2.1.28 additional information Salmonella enterica produces a proteinaceous microcompartment for B12-dependent 1,2-propanediol utilization, Pdu MCP. The Pdu MCP consists of catabolic enzymes encased within a protein shell and its function is to sequester propionaldehyde, a toxic intermediate of 1,2-propanediol degradation, overview. A short N-terminal region of the medium subunit, PduD, is required for packaging the coenzyme B12-dependent diol dehydratase, PduCDE, into the lumen of the Pdu MCP. PduD subunit also mediates packaging of itself and other subunits of diol dehydratase, PduC and PduE, into the Pdu MCP Salmonella enterica