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Literature summary extracted from

  • Housley, N.A.; Winkler, H.H.; Audia, J.P.
    The Rickettsia prowazekii ExoU homologue possesses phospholipase A1 (PLA1), PLA2, and Lyso-PLA2 activities and can function in the absence of any eukaryotic cofactors in vitro (2011), J. Bacteriol., 193, 4634-4642.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.32 additional information addition of bovine liver superoxide dismutase 1, SOD1, resultes in an increased rate of RP534-catalyzed phospholipid hydrolysis, mechanism, overview Rickettsia prowazekii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.32 expresssion of His-tagged wild-type and mutant enzymes in Escherichia coli strain C41[DE3] Rickettsia prowazekii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.32 D264A site-directed mutagenesis, substrate specificity compared to the wild-type enzyme, overview Rickettsia prowazekii
3.1.1.32 S100A site-directed mutagenesis, substrate specificity compared to the wild-type enzyme, overview Rickettsia prowazekii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.32 additional information inhibition of RP534 PLA2 activity by nanomolar amounts of the potent, irreversible inhibitor methyl arachidonoyl fluorophosphonate and by the reversible inhibitor arachidonoyl trifluoromethylketone Rickettsia prowazekii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.32 additional information Rickettsia prowazekii RP534 is a bona fide PLA enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Rickettsia prowazekii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41[DE3] by nickel affinity chromatography Rickettsia prowazekii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 1-acyl-2-oleoyl-sn-glycerophosphocholine + H2O labeled at the sn-2 position with [14C]oleic acid Rickettsia prowazekii acyl-glycerophosphocholine + oleic acid
-
 ?
3.1.1.32 additional information RP534 is a bona fide PLA enzyme Rickettsia prowazekii ?
-
 ?
3.1.1.32 additional information the Rickettsia prowazekii RP534 protein possesses phospholipase A1, i.e. PLA1, PLA2, and Lyso-PLA2 activities and can function in the absence of any eukaryotic cofactors in vitro, overview. RP534 protein is a homologue of the Pseudomonas aeruginosa ExoU phospholipase A secreted cytotoxin Rickettsia prowazekii ?
-
 ?
3.1.1.32 phosphatidylcholine + H2O labeled at the sn-2 position with [14C]arachadonic acid Rickettsia prowazekii 1-acyl-2-oleoyl-sn-glycerophosphocholine + arachidonic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 More the enzyme belongs to the ExoU family of PLAs Rickettsia prowazekii
3.1.1.32 RP534 PLA
-
 Rickettsia prowazekii
3.1.1.32 RP534 protein
-
 Rickettsia prowazekii

General Information

EC Number General Information Comment Organism
3.1.1.32 evolution mechanisms of activation of the ExoU family of PLAs may be evolutionarily conserved Rickettsia prowazekii