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Literature summary extracted from

  • Schuermann, M.; Wuebbeler, J.H.; Grote, J.; Steinbuechel, A.
    Novel reaction of succinyl coenzyme A (succinyl-CoA) synthetase: activation of 3-sulfinopropionate to 3-Sulfinopropionyl-CoA in Advenella mimigardefordensis strain DPN7T during degradation of 3,3-dithiodipropionic acid (2011), J. Bacteriol., 193, 3078-3089.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.4 gene sucCD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)/pLysS Advenella mimigardefordensis
6.2.1.4 gene sucCD, expression in Escherichia coli strain BL21(DE3)/pLysS Advenella mimigardefordensis
6.2.1.5 gene sucCD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)/pLysS Advenella mimigardefordensis

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.4 additional information transposon mutagenesis and deletion of gene sucCD, accumulation of 3SP during 3,3'-dithiodipropionic acid degradation occurs in Tn5::mob-induced mutants of Advenella mimigardefordensis strain DPN7T disrupted in sucCD and in the defined deletion mutant Advenella mimigardefordensis DELTAsucCD Advenella mimigardefordensis
6.2.1.5 additional information transposon mutagenesis and deletion of gene sucCD, accumulation of 3SP during 3,3'-dithiodipropionic acid degradation occurs in Tn5::mob-induced mutants of Advenella mimigardefordensis strain DPN7T disrupted in sucCD and in the defined deletion mutant Advenella mimigardefordensis DELTAsucCD Advenella mimigardefordensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.5 0.045
-
 CoA pH 7.4, 30°C, recombinant sucCD Advenella mimigardefordensis
6.2.1.5 0.083
-
 ATP pH 7.4, 30°C, recombinant sucCD Advenella mimigardefordensis
6.2.1.5 0.143
-
 succinate pH 7.4, 30°C, recombinant sucCD Advenella mimigardefordensis
6.2.1.5 0.448
-
 Itaconate pH 7.4, 30°C, recombinant sucCD Advenella mimigardefordensis
6.2.1.5 0.818
-
 Itaconate pH 7.4, 30°C, recombinant sucCD Advenella mimigardefordensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.4 Mg2+ SucCDAm is Mg2+- or Mn2+-dependent Advenella mimigardefordensis
6.2.1.4 Mn2+ SucCDAm is Mg2+- or Mn2+-dependent Advenella mimigardefordensis
6.2.1.5 Mg2+ SucCDAm is Mg2+- or Mn2+-dependent Advenella mimigardefordensis
6.2.1.5 Mn2+ SucCDAm is Mg2+- or Mn2+-dependent Advenella mimigardefordensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.4 GTP + succinate + CoA Advenella mimigardefordensis SucCDAm is unspecific regarding ATP or GTP GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 GTP + succinate + CoA Advenella mimigardefordensis DPN7T SucCDAm is unspecific regarding ATP or GTP GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA Advenella mimigardefordensis SucCDAm is unspecific regarding ATP or GTP ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA Advenella mimigardefordensis DPN7T SucCDAm is unspecific regarding ATP or GTP ADP + phosphate + succinyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.4 Advenella mimigardefordensis B3TZD8 subunit beta; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
6.2.1.4 Advenella mimigardefordensis B3TZD9 subunit alpha; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
6.2.1.4 Advenella mimigardefordensis DPN7T B3TZD8 subunit beta; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
6.2.1.4 Advenella mimigardefordensis DPN7T B3TZD9 subunit alpha; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
6.2.1.5 Advenella mimigardefordensis B3TZD8 subunit beta; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
6.2.1.5 Advenella mimigardefordensis B3TZD9 subunit alpha; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
6.2.1.5 Advenella mimigardefordensis DPN7T B3TZD8 subunit beta; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-
6.2.1.5 Advenella mimigardefordensis DPN7T B3TZD9 subunit alpha; i.e. Tetrathiobacter mimigardefordensis, gene SucCDAm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.4 recombinant soluble enzyme from Escherichia coli strain BL21(DE3)/pLysS by anion exchange chromatography Advenella mimigardefordensis
6.2.1.5 recombinant soluble enzyme from Escherichia coli strain BL21(DE3)/pLysS by anion exchange chromatography Advenella mimigardefordensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.4 GTP + 3-sulfinopropionate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis GDP + phosphate + 3-sulfinopropionyl-CoA determination of 3-sulfinopropionyl-CoA structure by using liquid chromatography-electrospray ionization-mass spectrometry ?
6.2.1.4 GTP + 3-sulfinopropionate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T GDP + phosphate + 3-sulfinopropionyl-CoA determination of 3-sulfinopropionyl-CoA structure by using liquid chromatography-electrospray ionization-mass spectrometry ?
6.2.1.4 GTP + itaconate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis GDP + phosphate + itaconyl-CoA
-
 ?
6.2.1.4 GTP + itaconate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T GDP + phosphate + itaconyl-CoA
-
 ?
6.2.1.4 GTP + succinate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 GTP + succinate + CoA succinate is the best substrate, SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 GTP + succinate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.4 GTP + succinate + CoA succinate is the best substrate, SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T GDP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + 3-sulfinopropionate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis ADP + phosphate + 3-sulfinopropionyl-CoA determination of 3-sulfinopropionyl-CoA structure by using liquid chromatography-electrospray ionization-mass spectrometry ?
6.2.1.5 ATP + itaconate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis ADP + phosphate + itaconyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA succinate is the best substrate, SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis ADP + phosphate + succinyl-CoA
-
 ?
6.2.1.5 ATP + succinate + CoA SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis DPN7T ADP + phosphate + succinyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.4 SucCDAm
-
 Advenella mimigardefordensis
6.2.1.4 Succinyl coenzyme A synthetase
-
 Advenella mimigardefordensis
6.2.1.4 Succinyl-CoA synthetase
-
 Advenella mimigardefordensis
6.2.1.5 SucCDAm
-
 Advenella mimigardefordensis
6.2.1.5 Succinyl coenzyme A synthetase
-
 Advenella mimigardefordensis
6.2.1.5 Succinyl-CoA synthetase
-
 Advenella mimigardefordensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.4 30
-
 assay at Advenella mimigardefordensis
6.2.1.5 30
-
 assay at Advenella mimigardefordensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.4 7.4
-
 assay at Advenella mimigardefordensis
6.2.1.5 7.4
-
 assay at Advenella mimigardefordensis

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.4 GTP SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis
6.2.1.5 ATP SucCDAm is unspecific regarding ATP or GTP Advenella mimigardefordensis