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Literature summary extracted from

  • Kimura, Y.; Yoshimi, M.; Takata, G.
    Enzymatic and mutational analyses of a class II 3,5-cyclic nucleotide phosphodiesterase, PdeE, from Myxococcus xanthus (2011), J. Bacteriol., 193, 2053-2057.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.17 gene pdeE, sequence comparisons Myxococcus xanthus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.17 additional information construction of a pdeE disruption mutant, phenotype, overview Myxococcus xanthus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.17 Fe2+ strong inhibition at 1 mM Myxococcus xanthus
3.1.4.17 additional information the broad-spectrum phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, IBMX, has no effect on the activity of PdeE at 1 mM. No inhibition by theophylline at 1 mM, beta-glycerophosphate at 0.1 mM, and by orthovanadate Myxococcus xanthus
3.1.4.17 Zn2+ strong inhibition at 1 mM Myxococcus xanthus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.17 0.012
-
 adenosine 3',5'-cyclic phosphate pH 7.0, 55°C Myxococcus xanthus
3.1.4.17 0.025
-
 guanosine 3',5'-cyclic phosphate pH 7.0, 55°C Myxococcus xanthus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.17 Co2+ required, activates Myxococcus xanthus
3.1.4.17 Mn2+ required, activates Myxococcus xanthus
3.1.4.17 additional information no effect on enzyme activity by Mg2+ Myxococcus xanthus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.17 27600
-
 x * 27600, about, sequence calculation Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.17 Myxococcus xanthus
-
 gene pdeE
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.17 0.0207
-
 substrate cGMP, pH 7.0, 55°C Myxococcus xanthus
3.1.4.17 0.0309
-
 substrate cAMP, pH 7.0, 55°C Myxococcus xanthus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.17 adenosine 3',5'-cyclic phosphate + H2O
-
 Myxococcus xanthus adenosine 5'-phosphate
-
 ?
3.1.4.17 guanosine 3',5'-cyclic phosphate + H2O
-
 Myxococcus xanthus guanosine 5'-phosphate
-
 ?
3.1.4.17 additional information substrate specificity of PdeE, overview Myxococcus xanthus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.17 ? x * 27600, about, sequence calculation Myxococcus xanthus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.17 class II 3,5-cyclic nucleotide phosphodiesterase
-
 Myxococcus xanthus
3.1.4.17 PdeE
-
 Myxococcus xanthus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.17 55
-
 assay at Myxococcus xanthus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.17 7
-
 assay at Myxococcus xanthus

General Information

EC Number General Information Comment Organism
3.1.4.17 malfunction pdeE mutant exhibits delays in fruiting body and spore formation compared with the wild type when cultured on starvation medium. It also shows reduced enzyme activity Myxococcus xanthus