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Literature summary extracted from

  • Dhouib, R.; Laval, F.; Carriere, F.; Daffe, M.; Canaan, S.
    A monoacylglycerol lipase from Mycobacterium smegmatis involved in bacterial cell interaction (2010), J. Bacteriol., 192, 4776-4785.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.23 expressed in Escherichia coli Rosetta(DE3)/pLysS cells Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.23 S111A inactive Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.23 29882
-
x * 29882, calculated from amino acid sequence Mycolicibacterium smegmatis
3.1.1.23 30000
-
x * 30000, recombinant enzyme, SDS-PAGE Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.23 Mycolicibacterium smegmatis
-
-
-
3.1.1.23 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.23 Ni-NTA column chromatography and Superdex 200 gel filtration Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.23 80
-
recombinant enzyme, using monomyristin as substrate, at 37°C and at pH 8.0 in 2.5 mM Tris-HCl buffer, 150 mM NaCl, and 3 mM sodium taurodeoxycholate Mycolicibacterium smegmatis
3.1.1.23 143
-
recombinant enzyme, using monomyristin as substrate, at 37°C and at pH 8.0 in 2.5 mM Tris-HCl buffer, 150 mM NaCl, and 3 mM sodium taurodeoxycholate Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.23 monomyristin + H2O
-
Mycolicibacterium smegmatis glycerol + myristate
-
?
3.1.1.23 monomyristin + H2O
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 glycerol + myristate
-
?
3.1.1.23 monoolein + H2O
-
Mycolicibacterium smegmatis glycerol + oleate
-
?
3.1.1.23 monoolein + H2O
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 glycerol + oleate
-
?
3.1.1.23 additional information no activity with triacetin, tributyrin, trioctanoin, triolein, dioctanoin, dimyristin, monooctanoin, diolein, and Tween 80 Mycolicibacterium smegmatis ?
-
?
3.1.1.23 additional information no activity with triacetin, tributyrin, trioctanoin, triolein, dioctanoin, dimyristin, monooctanoin, diolein, and Tween 80 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.23 ? x * 30000, recombinant enzyme, SDS-PAGE Mycolicibacterium smegmatis
3.1.1.23 ? x * 29882, calculated from amino acid sequence Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.23 monoacylglycerol lipase
-
Mycolicibacterium smegmatis
3.1.1.23 MSMEG_0220
-
Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.23 8
-
-
Mycolicibacterium smegmatis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.23 4 9 highly stable from pH 6.5 to 9.0 during 1 h of incubation. The enzyme shows a residual activity of 50% after 1 h of incubation at pH 4.0 Mycolicibacterium smegmatis