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Literature summary extracted from

  • Otto, K.; Hofstetter, K.; Röthlisberger, M.; Witholt, B., Schmid, A.
    Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component flavin-diffusible monooxygenase (2004), J. Bacteriol., 186, 5292-5302.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.11 expression in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.11 FAD at a FAD concentration exceeding 0.015 mM, the styrene oxide formation rate decreases Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.11 0.38
-
Styrene pH 7.5, 37°C, recombinant enzyme Pseudomonas sp.
1.14.14.11 0.45
-
Styrene pH 7.5, 37°C, wild-type enzyme Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.11 additional information StyA does not contain Fe2+, Cu2+, Zn2+, Mn2+, Co2+, or Mg2+ Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.11 46350
-
2 * 46350, MALDI-TOF analysis Pseudomonas sp.
1.14.14.11 47000
-
2 * 47000, SDS-PAGE Pseudomonas sp.
1.14.14.11 80000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2 Pseudomonas sp. the epoxidation of the vinyl side chain of styrene catalyzed by a monooxygenase is the initial reaction in one microbial aerobic styrene degradation pathway (S)-2-phenyloxirane + FAD + H2O
-
?
1.14.14.11 styrene + FADH2 + O2 Pseudomonas sp. VLB120 the epoxidation of the vinyl side chain of styrene catalyzed by a monooxygenase is the initial reaction in one microbial aerobic styrene degradation pathway (S)-2-phenyloxirane + FAD + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.11 Pseudomonas sp.
-
-
-
1.14.14.11 Pseudomonas sp. VLB120
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.11
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.11 1.9
-
pH 7.5, 37°C, wild-type StyA Pseudomonas sp.
1.14.14.11 2.1
-
pH 7.5, 37°C, recombinant StyA Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.14.11 4°C, addition of 10% v/v glycerol and 1 mM pefabloc to the storage buffer improves enzyme stability, the activity decreases by 20% during the first 2 weeks, StyA retains 60% of its initial activity after 3 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.11 additional information the wild-type enzyme catalyzes the epoxidation of p-, alpha-, and beta-methylstyrene, 1,2-dihydronaphthalene, methyl phenyl sulfide, and 3-chlorostyrene at rates comparable to those achieved with the recombinant form of the enzyme Pseudomonas sp. ?
-
?
1.14.14.11 additional information the wild-type enzyme catalyzes the epoxidation of p-, alpha-, and beta-methylstyrene, 1,2-dihydronaphthalene, methyl phenyl sulfide, and 3-chlorostyrene at rates comparable to those achieved with the recombinant form of the enzyme Pseudomonas sp. VLB120 ?
-
?
1.14.14.11 styrene + FADH2 + O2 the epoxidation of the vinyl side chain of styrene catalyzed by a monooxygenase is the initial reaction in one microbial aerobic styrene degradation pathway Pseudomonas sp. (S)-2-phenyloxirane + FAD + H2O
-
?
1.14.14.11 styrene + FADH2 + O2 styrene is exclusively converted to S-styrene oxide. During the epoxidation reaction, no formation of a complex of StyA and StyB is observed, suggesting that electron transport between reductase and oxygenase occurs via a diffusing flavin. StyA activity was strongly influenced by the amount of StyB added. No epoxidation activity is observed for the StyAB system when FAD is replaced by FMN or riboflavin Pseudomonas sp. (S)-2-phenyloxirane + FAD + H2O
-
?
1.14.14.11 styrene + FADH2 + O2 the epoxidation of the vinyl side chain of styrene catalyzed by a monooxygenase is the initial reaction in one microbial aerobic styrene degradation pathway Pseudomonas sp. VLB120 (S)-2-phenyloxirane + FAD + H2O
-
?
1.14.14.11 styrene + FADH2 + O2 styrene is exclusively converted to S-styrene oxide. During the epoxidation reaction, no formation of a complex of StyA and StyB is observed, suggesting that electron transport between reductase and oxygenase occurs via a diffusing flavin. StyA activity was strongly influenced by the amount of StyB added. No epoxidation activity is observed for the StyAB system when FAD is replaced by FMN or riboflavin Pseudomonas sp. VLB120 (S)-2-phenyloxirane + FAD + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.11 dimer 2 * 47000, SDS-PAGE Pseudomonas sp.
1.14.14.11 dimer 2 * 46350, MALDI-TOF analysis Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.14.11 StyA
-
Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.11 37
-
assay at Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.11 1.6
-
Styrene pH 7.5, 37°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.11 7.5
-
assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.11 FADH2 no epoxidation activity is observed for the StyAB system when FAD is replaced by FMN or riboflavin. At a FAD concentration exceeding 0.015 mM, the styrene oxide formation rate decreases Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.11 Pseudomonas sp. calculated from sequence
-
5.3