EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.11 | expression in Escherichia coli | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.11 | FAD | at a FAD concentration exceeding 0.015 mM, the styrene oxide formation rate decreases | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.11 | 0.38 | - |
Styrene | pH 7.5, 37°C, recombinant enzyme | Pseudomonas sp. | |
1.14.14.11 | 0.45 | - |
Styrene | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.11 | additional information | StyA does not contain Fe2+, Cu2+, Zn2+, Mn2+, Co2+, or Mg2+ | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.11 | 46350 | - |
2 * 46350, MALDI-TOF analysis | Pseudomonas sp. |
1.14.14.11 | 47000 | - |
2 * 47000, SDS-PAGE | Pseudomonas sp. |
1.14.14.11 | 80000 | - |
gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.11 | styrene + FADH2 + O2 | Pseudomonas sp. | the epoxidation of the vinyl side chain of styrene catalyzed by a monooxygenase is the initial reaction in one microbial aerobic styrene degradation pathway | (S)-2-phenyloxirane + FAD + H2O | - |
? | |
1.14.14.11 | styrene + FADH2 + O2 | Pseudomonas sp. VLB120 | the epoxidation of the vinyl side chain of styrene catalyzed by a monooxygenase is the initial reaction in one microbial aerobic styrene degradation pathway | (S)-2-phenyloxirane + FAD + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.11 | Pseudomonas sp. | - |
- |
- |
1.14.14.11 | Pseudomonas sp. VLB120 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.11 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.11 | 1.9 | - |
pH 7.5, 37°C, wild-type StyA | Pseudomonas sp. |
1.14.14.11 | 2.1 | - |
pH 7.5, 37°C, recombinant StyA | Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.14.11 | 4°C, addition of 10% v/v glycerol and 1 mM pefabloc to the storage buffer improves enzyme stability, the activity decreases by 20% during the first 2 weeks, StyA retains 60% of its initial activity after 3 months | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.11 | additional information | the wild-type enzyme catalyzes the epoxidation of p-, alpha-, and beta-methylstyrene, 1,2-dihydronaphthalene, methyl phenyl sulfide, and 3-chlorostyrene at rates comparable to those achieved with the recombinant form of the enzyme | Pseudomonas sp. | ? | - |
? | |
1.14.14.11 | additional information | the wild-type enzyme catalyzes the epoxidation of p-, alpha-, and beta-methylstyrene, 1,2-dihydronaphthalene, methyl phenyl sulfide, and 3-chlorostyrene at rates comparable to those achieved with the recombinant form of the enzyme | Pseudomonas sp. VLB120 | ? | - |
? | |
1.14.14.11 | styrene + FADH2 + O2 | the epoxidation of the vinyl side chain of styrene catalyzed by a monooxygenase is the initial reaction in one microbial aerobic styrene degradation pathway | Pseudomonas sp. | (S)-2-phenyloxirane + FAD + H2O | - |
? | |
1.14.14.11 | styrene + FADH2 + O2 | styrene is exclusively converted to S-styrene oxide. During the epoxidation reaction, no formation of a complex of StyA and StyB is observed, suggesting that electron transport between reductase and oxygenase occurs via a diffusing flavin. StyA activity was strongly influenced by the amount of StyB added. No epoxidation activity is observed for the StyAB system when FAD is replaced by FMN or riboflavin | Pseudomonas sp. | (S)-2-phenyloxirane + FAD + H2O | - |
? | |
1.14.14.11 | styrene + FADH2 + O2 | the epoxidation of the vinyl side chain of styrene catalyzed by a monooxygenase is the initial reaction in one microbial aerobic styrene degradation pathway | Pseudomonas sp. VLB120 | (S)-2-phenyloxirane + FAD + H2O | - |
? | |
1.14.14.11 | styrene + FADH2 + O2 | styrene is exclusively converted to S-styrene oxide. During the epoxidation reaction, no formation of a complex of StyA and StyB is observed, suggesting that electron transport between reductase and oxygenase occurs via a diffusing flavin. StyA activity was strongly influenced by the amount of StyB added. No epoxidation activity is observed for the StyAB system when FAD is replaced by FMN or riboflavin | Pseudomonas sp. VLB120 | (S)-2-phenyloxirane + FAD + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.11 | dimer | 2 * 47000, SDS-PAGE | Pseudomonas sp. |
1.14.14.11 | dimer | 2 * 46350, MALDI-TOF analysis | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.11 | StyA | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.11 | 37 | - |
assay at | Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.11 | 1.6 | - |
Styrene | pH 7.5, 37°C | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.11 | 7.5 | - |
assay at | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.11 | FADH2 | no epoxidation activity is observed for the StyAB system when FAD is replaced by FMN or riboflavin. At a FAD concentration exceeding 0.015 mM, the styrene oxide formation rate decreases | Pseudomonas sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.14.14.11 | Pseudomonas sp. | calculated from sequence | - |
5.3 |