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Literature summary extracted from

  • Umeno, D.; Arnold, F.
    Evolution of a pathway to novel long-chain carotenoids (2004), J. Bacteriol., 186, 1531-1536.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.96
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 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.96 A726G, A850G mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 F26L mutation is sufficient to gain phytoene synthase activity, decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 F26L/E149G mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 F26L/F267S mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 F26L/V43M mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 F26S/I40T mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 H12R/F26L/D27G/90G/K97R/H207R mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 K20E/F26S mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 M4V/H12R/F59S/Q81R/E180G mutant with phytoene synthase activity, without decrease in dehydrosqualene synthase activity Staphylococcus aureus
2.5.1.96 additional information construction of C40 carotenoid synthase pathway in Escherichia coli by expression of crtE encoding GGPP synthase, crtB encoding phytoene synthase, and crtI encoding phytoene desaturase, all from Erwinia uredovora. Replacement of phytoene synthase crtB by dehydrosqualene synthase crtM results in negligible ability to synthesize the C40 product. Some crtM mutants perform comparably to crtB in an in vivo C40 pathway. These mutants show significant variation in performance in their original C30 pathway, indicating the emergence of enzymes with broadened substrate specificity as well as those with shifted specificity Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.96 Staphylococcus aureus
-
-
-
2.5.1.96 Staphylococcus aureus ATCC 35556
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-
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