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Literature summary extracted from

  • Raisig, A.; Sandmann, G.
    4,4'-diapophytoene desaturase: catalytic properties of an enzyme from the C30 carotenoid pathway of Staphylococcus aureus (1999), J. Bacteriol., 181, 6184-6187.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.2 expression in Escherichia coli Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.2 0.01
-
 all-trans-4,4'-diapophytofluene pH and temperature not specified in the publication Staphylococcus aureus
1.3.8.2 0.049
-
 15-cis-4,4'-diapophytoene pH and temperature not specified in the publication Staphylococcus aureus
1.3.8.2 0.245
-
 all-trans-4,4'-diapo-zeta-carotene pH and temperature not specified in the publication Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.2 membrane it can be assumed that diapophytoene desaturase forms a complex in the membrane that keeps the substrate carotenoids bound until all three double bonds are introduced in the desaturation process Staphylococcus aureus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.2 52000
-
 1 * 52000, calculated from sequence Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.2 15-cis-4,4'-diapophytoene + 3 FAD Staphylococcus aureus the enzyme is involved in the C30 carotenoid pathway all-trans-4,4'-diaponeurosporene + 3 FADH2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.2 Staphylococcus aureus O07855
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.2
-
 Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.2 0.005
-
 pH and temperature not specified in the publication Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.2 15-cis-4,4'-diapophytoene + 3 FAD
-
 Staphylococcus aureus all-trans-4,4'-diaponeurosporene + 3 FADH2
-
 ?
1.3.8.2 15-cis-4,4'-diapophytoene + 3 FAD the enzyme is involved in the C30 carotenoid pathway Staphylococcus aureus all-trans-4,4'-diaponeurosporene + 3 FADH2
-
 ?
1.3.8.2 15-cis-4,4'-diapophytoene + FAD
-
 Staphylococcus aureus all-trans-4,4'-diapophytofluene + FADH2
-
 ?
1.3.8.2 all-trans-4,4'-diapo-zeta-carotene + FAD
-
 Staphylococcus aureus all-trans-4,4'-diaponeurosporene + FADH2
-
 ?
1.3.8.2 all-trans-4,4'-diapophytofluene + FAD
-
 Staphylococcus aureus all-trans-4,4'-diapo-zeta-carotene + FADH2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.2 monomer 1 * 52000, calculated from sequence Staphylococcus aureus

General Information

EC Number General Information Comment Organism
1.3.8.2 physiological function the enzyme is involved in the C30 carotenoid pathway Staphylococcus aureus