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Literature summary extracted from

  • Marzocca, M.P.; Harding, N.E.; Petroni, E.A.; Cleary, J.M.; Ielpi, L.
    Location and cloning of the ketal pyruvate transferase gene of Xanthomonas campestris (1991), J. Bacteriol., 173, 7519-7524.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.95 expression in Escherichia coli Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.95 phosphoenolpyruvate + D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol Xanthomonas campestris the enzyme is involved in the biosynthesis of the polysaccharide xanthan. 30-40% of xanthan terminal mannose residues of xanthan have a 4,6-O-(1-carboxyethan-1,1-diyl) ketal group 4,6-CH3(COO-)C-D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol + phosphate
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Organism

EC Number Organism UniProt Comment Textmining
2.5.1.95 Xanthomonas campestris
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.95 additional information also acts on the 6-O-acetyl derivative of the inner mannose unit Xanthomonas campestris ?
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 ?
2.5.1.95 phosphoenolpyruvate + D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
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 Xanthomonas campestris 4,6-CH3(COO-)C-D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol + phosphate
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 ?
2.5.1.95 phosphoenolpyruvate + D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol the enzyme is involved in the biosynthesis of the polysaccharide xanthan. 30-40% of xanthan terminal mannose residues of xanthan have a 4,6-O-(1-carboxyethan-1,1-diyl) ketal group Xanthomonas campestris 4,6-CH3(COO-)C-D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol + phosphate
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 ?

General Information

EC Number General Information Comment Organism
2.5.1.95 physiological function the enzyme is involved in the biosynthesis of the polysaccharide xanthan. 30-40% of xanthan terminal mannose residues of xanthan have a 4,6-O-(1-carboxyethan-1,1-diyl) ketal group Xanthomonas campestris