EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.73 | gene fae1A, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) | Anaeromyces mucronatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.73 | additional information | Fae1A is not affected by the addition of EDTA | Anaeromyces mucronatus | |
3.1.1.73 | phenylmethylsulfonyl fluoride | - |
Anaeromyces mucronatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.0052 | - |
ethyl ferulate | pH 7.2, 37°C | Anaeromyces mucronatus | |
3.1.1.73 | 0.0067 | - |
methyl ferulate | pH 7.2, 37°C | Anaeromyces mucronatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Anaeromyces mucronatus | F2YCB6 | gene fae1A | - |
3.1.1.73 | Anaeromyces mucronatus YE505 | F2YCB6 | gene fae1A | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | 2-naphthyl acetate + H2O | - |
Anaeromyces mucronatus | 2-naphthol + acetate | - |
? | |
3.1.1.73 | 2-naphthyl acetate + H2O | - |
Anaeromyces mucronatus YE505 | 2-naphthol + acetate | - |
? | |
3.1.1.73 | ethyl ferulate + H2O | - |
Anaeromyces mucronatus | ferulic acid + ethanol | - |
? | |
3.1.1.73 | ethyl ferulate + H2O | - |
Anaeromyces mucronatus YE505 | ferulic acid + ethanol | - |
? | |
3.1.1.73 | methyl ferulate + H2O | - |
Anaeromyces mucronatus | ferulic acid + methanol | - |
? | |
3.1.1.73 | methyl ferulate + H2O | - |
Anaeromyces mucronatus YE505 | ferulic acid + methanol | - |
? | |
3.1.1.73 | additional information | Fae1A exhibist activity against a number of substrates including naphthyl fatty acid esters, 4nitrophenyl fatty acid esters and hydroxylcinnamic acid esters, it has a higher affinity for ethyl and methyl ferulate than for the acetyl esters. It releases ferulic acid and 4-coumaric acid from barley straw. A serine residue plays a role in its activity | Anaeromyces mucronatus | ? | - |
? | |
3.1.1.73 | additional information | Fae1A exhibist activity against a number of substrates including naphthyl fatty acid esters, 4nitrophenyl fatty acid esters and hydroxylcinnamic acid esters, it has a higher affinity for ethyl and methyl ferulate than for the acetyl esters. It releases ferulic acid and 4-coumaric acid from barley straw. A serine residue plays a role in its activity | Anaeromyces mucronatus YE505 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | Fae1A | - |
Anaeromyces mucronatus |
3.1.1.73 | ferulic acid esterase | - |
Anaeromyces mucronatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 37 | - |
- |
Anaeromyces mucronatus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 15 | 37 | activity is optimal at 37°C, but 60% of activity is retained at 15°C. No activity is observed at 45°C | Anaeromyces mucronatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 7.2 | - |
- |
Anaeromyces mucronatus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 5.5 | 8 | over 50% activity within this range | Anaeromyces mucronatus |