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Literature summary extracted from

  • Szilagyi, M.; Kwon, N.J.; Dorogi, C.; Pocsi, I.; Yu, J.H.; Emri, T.
    The extracellular beta-1,3-endoglucanase EngA is involved in autolysis of Aspergillus nidulans (2010), J. Appl. Microbiol., 109, 1498-1508.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 91000
-
 2 * 91000, SDS-PAGE Aspergillus nidulans
3.2.1.39 230000
-
 PAGE Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 additional information Aspergillus nidulans enzyme is involved in the autolytic cell wall degradation resulting from carbon starvation of the fungus and liberates reducing sugars from cell debris ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Aspergillus nidulans
-
 member of glycosyl hydrolase family 81
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 from carbon-starving culture Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 9.1
-
 pH 6.5, temperature not specified in the publication Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Aspergillus nidulans ?
-
 ?
3.2.1.39 additional information enzyme is involved in the autolytic cell wall degradation resulting from carbon starvation of the fungus and liberates reducing sugars from cell debris Aspergillus nidulans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 dimer 2 * 91000, SDS-PAGE Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 EngA
-
 Aspergillus nidulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.39 45 65
-
 Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 55
-
 in absence of laminarin, unstable above Aspergillus nidulans
3.2.1.39 95
-
 in presence of laminarin, stable up to Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 6.5
-
-
 Aspergillus nidulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 3.5 10.5
-
 Aspergillus nidulans

Expression

EC Number Organism Comment Expression
3.2.1.39 Aspergillus nidulans regulation of engA is dependent on the FluG /BrlA asexual sporulation signalling pathway in submerged culture additional information

General Information

EC Number General Information Comment Organism
3.2.1.39 physiological function enzyme is involved in the autolytic cell wall degradation resulting from carbon starvation of the fungus. The deletion of either engA or chiB encoding an endochitinase, causes highly reduced production of hydrolases in general Aspergillus nidulans