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Literature summary extracted from

  • Lim, Y.R.; Yoon, R.Y.; Seo, E.S.; Kim, Y.S.; Park, C.S.; Oh, D.K.
    Hydrolytic properties of a thermostable alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus (2010), J. Appl. Microbiol., 109, 1188-1197.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Escherichia coli ER2566 cells Caldicellulosiruptor saccharolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Ca2+ 92% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
3.2.1.55 Co2+ 90% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
3.2.1.55 Cu2+ 79% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
3.2.1.55 EDTA 90% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
3.2.1.55 Fe2+ 83% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
3.2.1.55 Mg2+ 90% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
3.2.1.55 Mn2+ 97% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
3.2.1.55 additional information not inhibited by Ba2+ Caldicellulosiruptor saccharolyticus
3.2.1.55 Zn2+ 94% residual activity at 1 mM Caldicellulosiruptor saccharolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 1.1
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus
3.2.1.55 1.29
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 57882
-
 8 * 57882, calculated from amino acid sequence Caldicellulosiruptor saccharolyticus
3.2.1.55 58000
-
 8 * 58000, SDS-PAGE Caldicellulosiruptor saccharolyticus
3.2.1.55 460000
-
 gel filtration Caldicellulosiruptor saccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Caldicellulosiruptor saccharolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 heat treatment and Hi-Trap anion exchange column chromatography Caldicellulosiruptor saccharolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 1.1
-
 crude extract, in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus
3.2.1.55 28.2
-
 after 25.6fold purification, in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
3.2.1.55 additional information no activity towards 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-lactopyranoside, 4-nitrophenyl beta-D-maltopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, 2-nitrophenyl-beta-D-fucopyranoside, 2-nitrophenyl-beta-D-galactopyranoside and 2-nitrophenyl-beta-D-glucopyranoside, starch, arabinan, xylan, debranched arabinan and wheat arabinoxylan Caldicellulosiruptor saccharolyticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 homooctamer 8 * 58000, SDS-PAGE Caldicellulosiruptor saccharolyticus
3.2.1.55 homooctamer 8 * 57882, calculated from amino acid sequence Caldicellulosiruptor saccharolyticus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
 Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 80
-
-
 Caldicellulosiruptor saccharolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 65 80 the half-lives of the enzyme at 65°C, 70°C, 75°C and 80°C were 1700 h, 390 h, 49 h and 0.4 h, respectively Caldicellulosiruptor saccharolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 226
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus
3.2.1.55 285
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5.5
-
-
 Caldicellulosiruptor saccharolyticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.55 205
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus
3.2.1.55 221
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus