Literature summary extracted from

Vasur, J.; Kawai, R.; Jonsson, K.H.; Widmalm, G.; Engstroem, A.; Frank, M.; Andersson, E.; Hansson, H.; Forsberg, Z.; Igarashi, K.; Samejima, M.; Sandgren, M.; Stahlberg, J.
Synthesis of cyclic beta-glucan using laminarinase 16A glycosynthase mutant from the basidiomycete Phanerochaete chrysosporium (2010), J. Am. Chem. Soc., 132, 1724-1730.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.2.1.6	X-ray crystallography screening of ligands bound nucleophile mutant E115S, to 1 A resolution. Laminariheptaose binds in an arch with the reducing and nonreducing ends occupying either side of the catalytic cleft of the enzyme. alpha-Laminariheptaosyl fluoride can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan. The cyclic laminariheptaose molecule is not completely planar and torsion angles at the glycosidic linkages fluctuate between two energy minima	Phanerodontia chrysosporium

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.2.1.6	E115S	X-ray crystallography screening of ligands bound nucleophile mutant E115S. Laminariheptaose binds in an arch with the reducing and nonreducing ends occupying either side of the catalytic cleft of the enzyme. R-laminariheptaosyl fluoride can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan	Phanerodontia chrysosporium

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.6	Phanerodontia chrysosporium	Q874E3	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.6	alpha-laminariheptaosyl fluoride	-	Phanerodontia chrysosporium	?	substrate can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan. NMR confirms uniquely beta-1,3-linkages and no reducing end. The cyclic laminariheptaose molecule is not completely planar and torsion angles at the glycosidic linkages fluctuate between two energy minima	?
3.2.1.6	laminariheptaose + H2O	-	Phanerodontia chrysosporium	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.6	laminarinase 16A	-	Phanerodontia chrysosporium

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Release 2023.1 (January 2023)