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Literature summary extracted from

  • Yuan, Y.; Chung, H.S.; Leimkuhler, C.; Walsh, C.T.; Kahne, D.; Walker, S.
    In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides (2005), J. Am. Chem. Soc., 127, 14128-14129.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.278 medicine the activated enzyme is capable of transferring an alternative unnatural sugar donor. Because the desosamine sugar contacts the 50S subunit of ribosome, the discovery that an unnatural sugar can be transferred has implications for the synthesis of new erythromycin derivatives with improved properties. Reconstitute of the activity of EryCIII in vitro for the synthesis of unnatural macrolides Saccharopolyspora erythraea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.278 expression in Escherichia coli BL21(DE3) as a C-terminal His6 fusion protein Saccharopolyspora erythraea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.278 0.05
-
 3-alpha-mycarosylerythronolide B pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose Saccharopolyspora erythraea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.278 dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B Saccharopolyspora erythraea dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. dTDP-D-desosamine dTDP + erythromycin D
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.278 Saccharopolyspora erythraea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.278
-
 Saccharopolyspora erythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.278 dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. dTDP-D-desosamine Saccharopolyspora erythraea dTDP + erythromycin D
-
 ?
2.4.1.278 dTDP-D-mycaminose + 3-alpha-mycarosylerythronolide B the activated enzyme is capable of transferring an alternative sugar donor Saccharopolyspora erythraea dTDP + erythromycin M
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.278 EryCIII
-
 Saccharopolyspora erythraea
2.4.1.278 glycosyltransferase EryCIII
-
 Saccharopolyspora erythraea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.278 0.017
-
 3-alpha-mycarosylerythronolide B pH and temperature not specified in the publication, 0.05 mM TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose Saccharopolyspora erythraea

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.278 AKnT protein the noncognate glycosyltransferase activator protein AknT, stably activates EryCIII. Addition of purified AknT to inactive EryCIII restores the activity of EryCIII in vitro Saccharopolyspora erythraea
2.4.1.278 EryCII protein glycosyltransferase activator protein EryCII activates EryCIII. EryCIII treated with EryCII is catalytically active, effecting transfer of desosamine to 3-alpha-mycarosylerythronolide. The untreated EryCIII sample shows no activity Saccharopolyspora erythraea

General Information

EC Number General Information Comment Organism
2.4.1.278 physiological function the enzyme is involved in erythromycin biosynthesis Saccharopolyspora erythraea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.278 0.34
-
 3-alpha-mycarosylerythronolide B pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose Saccharopolyspora erythraea