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Literature summary extracted from

  • Mishina, Y.; Chen, L.X.; He, C.
    Preparation and characterization of the native iron(II)-containing DNA repair AlkB protein directly from Escherichia coli (2004), J. Am. Chem. Soc., 126, 16930-16936.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.33 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.33 D133C the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme Escherichia coli
1.14.11.33 H131C the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme Escherichia coli
1.14.11.33 H187C the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.33 Fe2+ the enzyme contains iron(II) Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.33 DNA-base-CH3 + 2-oxoglutarate + O2 Escherichia coli
-
 DNA-base + formaldehyde + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.33 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.33 DEAE-cellulose column chromatography, SP Sepharose cation exchange column chromatography, and Mono-S cation exchange column chromatography Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.14.11.33 the iron(II)-bound AlkB is reasonably stable at 4°C in the presence of 10 mM 2-mercaptoethanol at concentrations up to 2 mM Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2
-
 Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-base-CH3 + 2-oxoglutarate + O2
-
 Escherichia coli DNA-base + formaldehyde + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.33 AlkB
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.33 2-oxoglutarate
-
 Escherichia coli